| The purpose of this study was to provide a potentially novel approach to treat bone osteosarcoma using a multi-purpose scaffold combining local drug delivery and tissue regeneration functions.We have designed slowly-releasing microspheres delivering chemotherapy drug Adriamycin(ADM),and a decellularized periosteum scafforld to carry these microspheres.The decellularized periosteum(D-periosteum)were obtained by physical and chemical decellularization.Histological results showed that the cellular components were effectively removed.The D-periosteum showed excellent cytocompatibility,and the ability of promoting adhesion and growth of fibroblasts.Two kinds of slowly-releasing microspheres,the Adriamycin gelatin microspheres(ADM-GMS)and Adriamycin Poly(DL-lactide-co-glycolide)-gelatin microspheres(ADM-PLGA-GMS)were prepared and anchored to D-periosteum,resulting in two types of drug releasing regenerative scaffolds.The potential therapeutic effectiveness of these two scaffolds were tested in vitro by culturing human osteosarcoma cells with the scaffolds and evaluate the cellular viability overtime.In summary,a biologic material designed for local delivery of chemotherapy drugs has been demonstrated.Our results suggest these thereapetutic scaffolds can be used as a naturally biocompatible scaffold in future chemotherapy. |
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