Effects Of Interfering The Expression Of Insulin-like Growth Factor-binding Protein Related Protein 1 On Biological Behaviors Of Nasopharyngeal Carcinoma Cell Line

Objective: To obverse the changes of biological behaviors of nasopharyngeal carcinoma cell line CNE1 and CNE-2Z after the expression of insulin-like growth factor-binding protein related protein 1(IGFBP-rP1)gene was inhibited by RNA interference,and to explore the possible molecular mechanisms.Methods: The expression of IGFBP-rP1 in 25 cases of nasopharyngeal carcinoma tissues and 22 cases of chronic nasopharyngitis tissues was measured by immunohistochemical method.The specific siRNAs targeting IGFBP-rP1 gene(IGFBP-rP1 siRNAs)and the negative control RNA were transfected into nasopharyngeal carcinoma cell line CNE1 and CNE-2Z,respectively.Then the efficacy of IGFBP-rP1gene-siliencing was verified by real-time fluorescent quantitative PCR and Western blotting.Meanwhile,the expressions of total extracellular regulated kinase(ERK)1/2 and phosphorylated ERK(p-ERK)were detected by Western blotting.Then the migration,invasion andproliferation of CNE1 and CNE-2Z cells transfected with IGFBP-rP1 siRNAs were examined by cell scratch healing assay,Transwell invasion assay and CCK-8 assys,respectively.Results: The expression level of IGFBP-rP1 protein in nasopharyngeal carcinoma tissues was higher than that in chronic nasopharyngitis tissues(P < 0.05).After transfection with IGFBP-rP1 siRNA,the expression of IGFBP-rP1 in CNE1 and CNE-2Z cells was significantly down-regulated(both P < 0.05);the cell migration,invasion and proliferation of CNE1 and CNE-2Z cells were significantly inhibited(both P < 0.05);the expression level of p-ERK was significantly decreased(both P < 0.05),while the expression level of total ERK1/2 was unchanged in CNE1 and CNE-2Z cells(both P > 0.05).Conclusion: Down-regulation of IGFBP-rP1 gene expression maybe inhibit he proliferation,migration and invasion of nasopharyngeal carcinoma cells by regulating the ERK pathway.It is suggested that IGFBP-rP1 probably become a new target for the gene thrapy of nasopharyngeal carcinoma.