Establishment And Analysis Of Nasopharyngeal Carcinoma Cells CNE-1 With DEPDC1 Stable Silencing

Objective: The present study aimed to investigate the role of DEPDC1(DEP domain containing 1)in the pathogenesis of nasopharyngeal carcinoma.Methods: The expression level of DEPDC1 in archival tissue samples from nasopharyngeal carcinoma patients and healthy individuals was examined by quatitative RT-PCR and immunohistochemical assay.The nasopharyngeal carcinoma cell line CNE-1 with stable suppression of DEPDC1 expression was established by short hairpin RNA,and their proliferative capability and cell cycle status were separately assessed by CCK-8 assay and FACS assay.Additionally,their migratory and invasive abilities were detected using wound healing assay,transwell migration and invasion assays.Meanwhile,tumor formation assays in nude mice detected tumorigenicity in vivo.Results: The expression level of DEPDC1 in the tumor tissues(0.699±0.521)was significantly higher than that in the normal tissues(0.408±0.183)(P<0.05).Suppression of DEPDC1 in CNE-1 cells revealed significant inhibition of cellular proliferation as well as G2/M phase arrest.Depletion of DEPDC1 also remarkably attenuated cell motility and invasiveness(,accompanied by the reduction of Twist1(0.710±0.034)and Vimentin(0.780±0.063)(P<0.05).Knockdown of DEPDC1 in CNE-1 stable cell lines cause an obvious decrease in tumor weight and volume(0.23±0.03 g,46.91±15.07mm3)compared with the control group(0.56±0.17 g,546.24±93.46mm3)(P<0.05).Conclusion: Our findings have uncovered a novel role for DEPDC1 in cell cycle progression and metastasis of nasopharyngeal carcinoma,and provide a rationale for its potential in the diagnosis and/or therapy of nasopharyngeal carcinoma.