LOX-5/COX-2 Expression And Its Correlation With Uterine Adenomyosis Inflammatory Pathological Study

Objective: Adenomyosis is a common disease and a chronic inflammatory disease in women of childbearing age.leukotriene B4 and prostaglandin PGE2,arachidonic acid metabolites catalyzed by LOX-5 / COX-2,are important mediums of histological inflammatory response and inflammatory proliferation of targeted cell,playing important roles in the pathological mechanism of endometriosis.The present study was designed to investigate the expression of LOX-5,COX-2,IL-6 and IL-8 in eutopic and ectopic endometrium of adenomyosis and to investigate the correlation between LOX-5/COX-2 and histopathological pathology.Methods: Specimens of eutopic endometrium(EU)and ectopic endometrium(EC)were collected from 20 patients with adenomyosis undergoing surgery at First Clinical Medical College of Fujian Medical University.Twenty cases of healthy contral endometrium(CE)were also collected for comparison.The m RNA and protein expression of LOX-5,COX-2,IL-6 and IL-8 in CE,EU,EC were detected by RT-PCR and Western blot.The correlation between LOX-5/COX-2 and IL-6/IL-8in different group were analysed by SPSS software.Results: Our data showed that the m RNA expression of IL-6 and IL-8 in EC and EU group was significantly higher than CE group(P < 0.01),and the EC group was significantly higher than EU group(P < 0.01).The m RNA expression of LOX-5 and COX-2 in EC and EU group was significantly higher than in the EC group(P < 0.01),and LOX-5 expressed in EC group was significantly higher than EU group(P < 0.01).Western blot showed that the expressiong of LOX-5 in EC group was significantly higher than CE group(P < 0.01)and EU group(P < 0.05);COX-2 in EC and EU group was significantly higher than CE group(P < 0.01).Pearson correlation analysis showed that the m RNA expression of LOX-5 and COX-2 were positively correlated with IL-6 and IL-8 both in EU and EC.Conclusion: Our study demonstrates that the LOX-5 and COX-2 are overexpressed in EU and EC,and have good correlation with IL-6 and IL-8.These suggest that LOX-5/COX-2-mediated inflammatory response may involve inflammatory pathology and development of adenomyosis.