Overexpression Of CILP2 Increases OxLDL Induced Foam Cell Formation Via CD36 And PPARγ

PART ONE EFFECTS OF OVEREXPRESSION OF CILP2 ON OXLDL INDUCED FOAM CELL FORMATIONObjective: To investigate the effect of CILP2 on the formation of foam cells induced by oxidized low density lipoprotein(LDL).Methods: After treated with PMA for 24 hours,THP-1 cells differentiated into macrophages.And oxidized low-density lipoprotein for24 hours to construct a foam cell model in vitro.Real-time quantitative PCR(RT-PCR)was used to detect the effect of Ad-CILP2 infection on macrophage.Effect of CILP2 on the Formation of Foam Cells Induced by Oxidized Low Density Lipoprotein was measured by Oil Red Staining.Result: Oil red staining showed that the in vitro model of foam cells was constructed successfully.The level of CILP2 m RNA in macrophages was significantly increased after Ad-CILP2 infection(P <0.01).And theformation of foam cells in Ad-CILP2+ox LDL group was significantly higher than that in Ad-GFP+ox LDL group.Conclusion: CILP2 increases oxidized low density lipoprotein induces foam cell formation.PART TWO THE MECHANISM OF CILP2 INCREASES OXIDIZED LOW DENSITY LIPOPROTEIN-INDUCED FOAM CELL FORMATIONObjective: To investigate the possible mechanism of CILP2 increasing the formation of foam cells induced by oxidized low density lipoprotein.Methods: Dil-ox LDL was used to treat macrophages,and the uptake of lipids was observed by fluorescence uptake microscope.The m RNA expressions of scavenger receptor CD36,LOX-1 and SR-A were detected by RT-PCR.The protein expression of CD36 was detected by western blot.The m RNA expression of ABCA1 and ABCG1 was detected by RT-PCR.The cells were pretreated with PPAR-γ agonists or inhibitors for 24 hours and the expression of CD36 and LOX-1 m RNA was detected by RT-PCR.Result: CILP2 increased Dil-ox LDL uptake.Compared with Ad-GFP group,the expression of CD36 and LOX-1 m RNA in Ad-CILP2 group was significantly higher than that in Ad-GFP group(P <0.01/P <0.05),However,CILP2 had no effect on the m RNA expression of SR-A,ABCA1 and ABCG1 CILP2 increased protein the expression of CD36(P <0.01).The expression of CD36 m RNA was increased in macrophages aftertreated with rosiglitazone(P <0.05)The expression of CD36 m RNA was decreased inmacrophages after treated with GW9662(P <0.05).Rosiglitazone and GW9662 had no effect on LOX-1 m RNA expressionConclusion: CILP2 increased lipid uptake and increased expression of CD36 in macrophages by activating PPAR-γ signaling pathway.