The Variation And Significance Of The Hypoxia Inducible Factor 1α In The Patients With AECOPD

Objective:(1)To investigate the expression and significance of hypoxia-inducible factor-1α(HIF-1 α)in COPD stable phase,AECOPD and healthy subjects;(2)To investigate the expression and significance of hypoxia-inducible factor-1α(HIF-1α)in all levels of AECOPD,and to explore the relationship between the expression of hypoxia-inducible factor-1α and COPD.(3)To investigate the correlation between hypoxia-inducible factor-1α(HIF-1α)and FEV1%,Hs-CRP,PO2 and PCO2 in patients with AECOPD,Explore the role of HIF-1α in the progression of the disease in AECOPD.Methods:1.The subjects were divided into three goups,group A was 120 patients with acute exacerbation of COPD(grade Ⅰ,grade Ⅱ,grade Ⅲ and grade Ⅳ).Group B was COPD 60 cases of stable stage,C group as the control group,composed of 60 healthy peers.Time from May 2016 to January 2017,COPD patients from the First People’s Hospital of Yinchuan City hospital or outpatient,the control group composed of 60 healthy peers.All the subjects were collected 5ml of peripheral venous blood,The HIF-1 α was measured by enzyme-linked immunosorbent assay(ELISA).Pulmonary function test: Forced expiratory volume(FVC)and the ratio of FVC and predicted value(FVC%),FEV1,FEV1% and FEV1/FVC were detected in the pulmonary functiontest.partial pressure of oxygen(PO2)and partial pressure of carbon dioxide(PCO2)were measured by arterial blood gas analysis.The levels of Hypersensitive C-reactive protein were Detected in the laboratory department in our hospital.Statistical methods: The data for statistical analysis of the data for the SPSS22.0 For Windows software package,the collected data refer to the research content group,each group ofcontinuous variable data using the mean ± standard deviation((?)± s)(p<0.05),and the correlation analysis was analyzed by Spearman method.The correlation between the two groups was analyzed by one-way ANOVA and q-test.Results :There was no significant difference in gender ratio,age,height and between the healthy control group,the COPD group and the AECOPD group(p> 0.05).There was no significant difference in sex,age,height and between the male and female in the AECOPD group(p> 0.05).The levels of HIF-1α,Hs-CRP and PCO2 in the AECOPD group,COPD stable group and healthy control group were significantly different(p<0.001).AECOPD >COPD>HEALTHY SUBJECTS.(p<0.05).(3)In the AECOPD group,the level of PO2 in the AECOPD group was much lower than that in the COPD group and the healthy control group(p<0.05),and the difference was statistically significant(p<0.05)The levels of HIF-1α and The expression level of Hs-CRP were correlated with the degree of AECOPD.the difference was statistically significant(p<0.05).FEV1% was negatively correlated with HIF-1α(r=-0.416,p<0.05),FEV1% Hs-CRP was negatively correlated(r=-0.63,p<0.05),FEV1% was positively correlated with PO2(r=0.425,p<0.05).FEV1% was negatively correlated with PCO2(r =-0.208,p<0.05).(r= 0.209,p<0.05).There was a negative correlation between HIF-1αand PO2(r =-0.198,p<0.05).HIF-1αwas not correlated with PCO2(r = 0.152,p> 0.05).Conclusion:1.The levels of HIF-1αin AECOPD group、COPD group were significantly increased.2.The levels of HIF-1αincreased with the increase of AECOPD grade.3.HIF-1αis associated with FEV1%,Hs-CRP and PO2 in patients with AECOPD,which can respond to hypoxia and inflammation in AECOPD patients.