| Objective:Endometrial Carcinoma is one of the major malignancies of th e female reproductive system,One of the most common histologic types is endometrioid adenocarcinoma.Inhibitor of apoptosis protein(IAPs)family members XIAP and Livin protein can inhibit caspases(Caspase)induced cell apoptosis,cell cycle regulation and other biological functions,and tumor development is closely related to the key molecules.The objective of our study by immunohistochemical method,is to detect the expression of XIAP and Livin in the normal endometrium,atypical hyperplastic endometrium and endometrial adenocarcinoma in three tissues,and explain the relationship between XIAP and Livin,so that we can guide the clinical diagnosis and treatment of endometrial cancer,and Improve survival rates for cancer patients.Methods: 120 patients treated at the first hospital of Hebei Medical University from October 2013 to March 2016,who have been removed from the uterus,40 with endometrial adenocarcinoma,40 with atypical hyperplastic endometrium,and 40 with normal endometrial tissue as a comparison.The expression of XIAP and Livin were detected by immunohistochemical SP method in three groups.The data were processed by SPSS13.0 statistical software,the count data was compared by X2 test for significant analysis,P<0.05 was considered statistically significance.Results:1 HE stainingThe experimental result shows :the cells in normal endometrial group is rule and neat;In the atypical hyperplastic endometrium tissues the cells polaritr was disorder,and has irregular arrangement;In endometrial adenocarcinoma group,the cells arranged extremely in disorder,and cell division could easily be seen.2 XIAP in each groupIt was in endometrial adenocarcinoma group and atypical hyperplastic endometrium group but not normal endometrial group that of which the XIAP was strongly positive.The expression rate of endometrial adenocarcinoma group to normal endometrial group was 77.5%(31/40),70%(28/40)and 20%(8/40)separately.The expression rate of XIAP in endometrial adenocarcinoma group and the atypical hyperplastic endometrium group was significantly higher than the normal endometrial group(P < 0.05),and the rate in endometrial adenocarcinoma group was higher than the atypical hyperplastic endometrium group,but there is no difference in statistics(P>0.05).3 Livin in each groupIt was in endometrial adenocarcinoma group and atypical hyperplastic endometrium group but not normal endometrial group that of which the Livin was strongly positive.The expression rate of endometrial adenocarcinoma group to normal endometrial group was 80%(32/40),75%(30/40)and22.5%(9/40)separately.The expression rate of Livin in endometrial adenocarcinoma group and the atypical hyperplastic endometrium group was significantly higher than the normal endometrial group(P<0.05),and the rate in endometrial adenocarcinoma group was higher than the atypical hyperplastic endometrium group,but there is no difference in statistics(P >0.05).4 The relationship between clinicopathological parameters and XIAP in endometrioid adenocarcinomaIn endometrioid adenocarcinoma group,the positive expression of XIAP was not significantly different from the age,depth of myometrial invasion and lymph node metastasis(P>0.05).In clinical stage,the positive rate of XIAP was 55.6%(5/9)in stage I patients,and 62.5%(5/8)in patients with stage II,with stage III and IV was 91.3%(21/23).Statistical analysis showed that the positive expression rate of XIAP was significantly different in the clinical stages(P<0.05).The positive rate of XIAP in patients with grade G1 was55.6%(5/9),in grade G2 was 57.1%(4/7);G3 was 91.7%(22/24).Statisticalanalysis showed that the positive expression rate of XIAP was significantly different in different pathological grades(P<0.05).5 The relationship between clinicopathological parameters and Livin protein in endometrioid adenocarcinomaIn 40 cases of endometrioid adenocarcinoma,the positive expression of Livin was not significantly different from the age,depth of myometrial invasion and lymph node metastasis(P>0.05).In clinical stage,the positive rate of Livin was 55.6%(5/9)in stage I patients,and 62.5%(5/8)in patients with stage II.with stage III and IV was 95.7%(22/23).Statistical analysis showed that the positive expression rate of Livin was significantly different in the clinical stages(P<0.05).The positive rate of Livin in patients with grade G1 was 55.6%(5/9).grade G2 was 57.1%(4/7);G3 was 95.8%(23/24).Statistical analysis showed that the positive expression rate of Livin was significantly different in different pathological grades(P<0.05).6 Relationship between XIAP and Livin in endometrioid adenocarcinomaIn endometrial adenocarcinoma of XIAP positive,the expression rate of Livin was 93.5%(29/31),in endometrioid adenocarcinoma of XIAP negative,the expression rate of Livin was 33.3%(3/9);in endometrial adenocarcinoma of Livin positive,the expression rate of XIAP was 90.6%(29/32);in endometrioid adenocarcinoma of Livin negative,the expression rate of XIAP was 25.0%(2/8).Statistical analysis showed that the expression rate of XIAP was positively correlated with the expression rate of Livin(P<0.05).Conclusions:1 Compared with normal endometrium,the expression of XIAP and Livin in endometrial adenocarcinoma and atypical hyperplastic endometrium was significantly increased.2 In endometrioid adenocarcinoma tissues,the positive expression of XIAP and Livin has no correlation with age,lymph node metastasis and the depth of invasion,but it has correlation with clinical staging and pathological classification.The higher the level of clinical staging and pathology,the higher the positive expression rate.3 In endometrial adenocarcinoma,the expression rate of XIAP was positively correlated with the expression rate of Livin. |
PDF Full Text Request