Relationship Between Vitamin D Receptor And 1,25-（OH）₂D₃ And Colorectal Cancer And Its Clinical Significance

Objective:To investigate the correlation between the level of vitamin D receptor and the content of 1,25-（OH）₂D₃ and colorectal cancer.For colorectal cancer patients to provide a better basis for diagnosis and treatment.Methods: Twenty patients with colorectal cancer diagnosed by pathology from January 2015 to January 2016 at the First Affiliated Hospital of Shihezi University were selected to meet the criteria of colorectal cancer patients according to the criteria of inclusion and exclusion.For example,each of the two experienced pathologists according to WHO（2011 version）digestive tract tumor classification criteria diagnosis and diagnosis,while patients from the malignant tumor tissue more than 10 cm normal colorectal tissue,as the control group.The levels of vitamin D receptor（VDR）in malignant tissues and normal tissues were detected by real-time quantitative PCR（qrt-PCR）.The levels of vitamin D receptor（VDR）in the patients were measured by ELISA（enzyme-linked immunosorbent assay）,25-（OH）₂D₃ levels,collecting and sorting the data,comparing the results and correlations.Results:（1）VDR can be detected in all tissues,and the amplification curve is good and the amplification efficiency is high.（2）The relative expression levels of VDR in colorectal tissues and healthy colorectal tissues were 5.72 ± 6.58 and 0.91 ± 5.98,respectively.According to the qRT-PCR principle,the higher the ΔCt value,the higher the expression level of the target gene in the corresponding tissues The expression of VDR in patients with colorectal malignancy was lower than that in the control group,and the difference between the two groups was less than 0.05,which was statistically significant.（3）There was no correlation between the relative expression of VDR and the level of 1,25-（OH）₂D₃ in the blood of patients with colorectal cancer.Conclusion:（1）.The expression of vitamin D receptor in colorectal malignancies is lower than that in normal colorectal tissues,suggesting that vitamin D receptors are associated with the development and progression of colorectal malignancies,but the expression of vitamin D receptors Whether the decline began before the occurrence of colorectal cancer,or colorectal cancer after the occurrence of vitamin D receptor decline is still to be further studied.（2）The expression of vitamin D receptor was positively correlated with the degree of differentiation of colorectal adenoma（P <0.05）.The expression of vitamin D receptor was positively correlated with the degree of differentiation of colorectal adenocarcinoma,The expression of vitamin D receptor and colorectal cancer patients with lymph node metastasis was positively correlated.That is,with the decrease of vitamin D receptor expression,colorectal cancer metastasis rate is higher.But there was no significant relationship between the age,sex,tumor stage,histological type,growth pattern and depth of invasion.The expression of vitamin D receptor could be used as the basis for judging the degree of tumor differentiation.As a basis for the evaluation of lymph node metastasis in patients with colorectal cancer.（3）There was no association between the expression of vitamin D receptor and the content of1,25-（OH）₂D₃ in peripheral blood of colorectal cancer patients in colorectal malignant tumor tissues.