Study On The Relationship Between High-risk HPV Infection And Immune Function In Uighur Women

Objective: Cervical cancer is one of the highly malignant tumors of Xinjiang Uighur women.Using several molecular biology and immunoassay methods to understand the high risk of HPV infection in Uighur women and risk factors.To explore the relationship between the development of HPV infection,the development of cervical cancer and the changes of multiple immune indexes and the correlation between the indicators,and provide the theoretical basis for follow-up immunotherapy.Methods: From December 2015 to September 2016,out patients or hospitalized women who were admitted to the People’s Hospital of Xinjiang Autonomous Region were voluntarily receiving HC2 test for HPV infection with or without combined liquid-based cytology(TCT).For HPV infection positive or liquid-based cells(TCT)diagnosis ≥ ASC,and further colposcopy and biopsy to send pathological inspection.Simultaneous,extraction of venous blood 2 to 3 ml,by flow cytometry detection of T lymphocyte subsets.Serum / plasma by enzyme-linked immunosorbent assay detection of human papillomavirus type 16 L1-IgG antibody levels.Results:1.(1)The positive rates of HPV in Uighur and Han were 87.93% and 68.04%respectively.There was significant difference between the two ethnic groups(P <0.01).(2)HPV load RLU / CO ≥ 1.0 is positive,<1 0 is negative,according to the HPV load is divided into <1,1 ~ 100,100 ~ 1000,1000< groups.There was a positive correlation between cervical lesions and HPV load(RLU / CO).The higher the HPV load,the more severe cervical lesions(P <0.01).(3)Uighur and Han both groups,HPV-negative(RLU / CO <1),respectively group1 ~100,group 100 ~ 1000,> 1000 group;and group 1 ~ 100 and> 1000;100 ~ 1000,and the group> 1000(P <0.05).(4)The results of liquid-based cytology(TCT)were divided into Negative For Intraepithelial Lesion Or Malignancy(NILM),atypical squamous cell of Undetermined signification(ASC-US),low-grade squamous intra epithelial lesion(LSIL),high-grade squamous intra epithelial lesion(HSIL),squamous carcinoma and Atypical glandular cells of undetermined significance(SCC+AGUS).There was a significantdifference between NILM,ASC-US,LSIL,HSIL,SCC + AGUS and HPV(P < 0.01).In the NILM group and the SCC + AGUS group,30.77%(4/13)of the Han patients and 69.23%(9/13)of the Uighur patients were different(P <0.01).(5)Age and HPV load were gradually increased,35 ~ 45 years old and 45 ~ 55 years old significantly increased(P <0.05).(6)Cervical lesions were closely related to age,and there was significant difference between the two groups(P <0.05).2.(1)The percentages of CD4 helper / inducer T cell were 33% ~ 58%,and they were divided into four groups according to the reference range: <33,33 ~ 45,45 ~ 58,> 58 and with the increase of cervical lesions,CD4 The percentage of T cells decreased significantly in the range of <33,33 ~ 45,the difference was statistically significant(P <0.01).(2)CD4 / CD8 ratio of 0.71 ~ 2.78,according to the reference range of high and low divided into <0.71,0.71 ~1.74,1.74 ~ 2.78,> 2.78 and other 4 groups;with the degree of cervical lesions increased CD4/ CD8 ratio of <0.71,0.71 ~1.74.The difference between the two groups was statistically significant(P <0.01).(3)Total CD3 T cell percentage reference range of 56% ~ 86%,according to the reference range of high and low divided into <56,56 ~ 71,71 ~ 86,> 86 and other 4 groups;with cervical lesions increased CD3 total T cell percentage 56 ~ 71,71 ~ 86,but the difference was not statistically significant(P> 0.05).(4)CD8 inhibitory / killer T cell percentage reference range of 13% ~39%,according to the reference range of high and low divided into <13,13 ~ 26,26 ~ 39,> 39 and other 4 groups;With cervical lesions increased CD8 inhibitory / killer T cell percentage of cells> 39 decreased,but the difference was not statistically significant(P> 0.05).3.(1)With the increase of cervical lesions,the positive rate of HPV 16 L1-IgG antibody decreased,but there was no significant difference between the two groups(P> 0.05).(2)There was no significant difference in HPV loading(RLU/CO)with HPV 16 L1-IgG antibody level(P> 0.05).(3)There was no correlation between HPV16 L1-IgG antibody level and different ethnic groups(P> 0.05).(4)There was no correlation between HPV16 L1-IgG antibody level and age(P> 0.05).Conclusion: 1.There was a positive correlation between cervical lesion and HR-HPVinfection rate.Increased HPV load is associated with high levels of cervical intraepithelial lesion and cervical cancer risk;cervical cancer occurs when the viral load is low,the reason may be related to HPV cervical epithelial cell necrosis.With the increase of cytology and pathological changes of cervical lesions,HR-HPV load and HPV positive rate were increased.2.The immune function of the body and immune dysfunction are increased risk of cervical lesions..The cellular immune function of patients with HPV positive high-grade cervical lesions was reduced to varying degrees,and the cellular immune function of patients with cervical cancer was the most significant.3.Serum HPV16 L1-IgG antibody test positive can represent the existing HPV infection,but also that the previous HPV infection.The positive rate of HPV 16 L1-IgG antibody decreased with the increase of cervical lesions,and the results may be related to the reversal of HPV clearance.