| Objective:1.Investigate the prevalence and drug sensitivity of Staphylococcus aureus in patients with chronic sinusitis(CRS)and non CRS patients,and to provide reference for the use of antimicrobial agents in the treatment of CRS.2.Compare the virulence genes of nasal Staphylococcus aureus in patients with chronic sinusitis with nasal polyps(CRSwNP)and chronic sinusitis without nasal polyps(CRSsNP),and to explore the relationship with different virulence genotypes infected with strains of sinusitis and nasal polyps.3.Identify the predominant type of Staphylococcus aureus in patients with CRSwNP/CRSsNP,to evaluate the epidemic trend,to explore the evolution and genetic characteristics of the strains,and to provide evidence for the prevention and control of the disease.Methods:1.Isolation and identification of Staphylococcus aureus: culture of nasal mucosal surface secretions were obtained from the control group and the experimental group(including CRSwNP and CRSsNP group),the cultivation of positive strains in VITEK2 automatic microorganism identification system identification.2.Drug sensitivity test: the strain of Staphylococcus aureus was identified by Kirby Bauer agar diffusion method(K-B method).3.Detection of virulence factors: detection of 17 virulence genes of the isolated strains of Staphylococcus aureus was carried out in 41 strains by PCR.4.MLST typing:7 kinds of housekeeping genes were amplified by PCR method,and then the sequences of the 7 housekeeping genes were spliced and analyzed by MEGA6.0 software.Results:1.The experimental group of Staphylococcus aureus positive rate was significantly higher than the control group(χ2=16.778,P=0.000 < 0.05);the experimental group in CRSwNP group and CRSsNP group of Staphylococcus aureus infection rate had no difference(χ2 =0.542,P=0.480>0.05).2.41 strains of Staphylococcus aureus detection to 10 kinds of antimicrobial drug resistant,which has the highest rate of resistance to penicillin,clindamycin,erythromycin,experimental group and control group of drug resistance was no difference(P>0.05)3.(1)The detection rate of SEB in the experimental group was significantly higher than the control group(χ2 =5.864,P=0.015<0.05);the detection rate of SEB in CRSwNP group was higher than that in group CRSsNP(P=0.009<0.05);the remaining 5 enterotoxin genes in the experimental group and the control group had no statistical significance(P > 0.05);(2)the cell wall-associated proteins of Staphylococcus aureus were detected in the experimental group and control group,and the detection rate is high,there was no significant difference between the experimental group and the control group(P>0.05);(3)HLα、HLβ gene encoding the hemolysin detection rate were high in the experimental group and control group,and the 2 genes in the experimental group and the control group had no statistical significance(P>0.05).4.41 strains of Staphylococcus aureus were found 17 different types of ST by MLST typing,ST59 type had a total of 8 strains,accounting for about 19.51% of all strains(8/41).In the experimental group,the most common type was ST59,accounting for 24.14%(7/29),followed by ST188,ST6,ST72,accounting for 10.34%(3/29).Conclusion:1.Some patients with CRS may be associated with Staphylococcus aureus infection.2.The resistance rate of Staphylococcus aureus isolated from nasal cavity was higher in this province;staphylococcus aureus sensitive to aminoglycosides,quinolones,glycopeptide antimicrobial;there was no difference in the prevalence of Staphylococcus aureus in patients with sinusitis and non CRS.3.Carrying enterotoxin gene of Staphylococcus aureus was more likely to cause CRS,and the relationship between strain infection and nasal polyps may carry more types of enterotoxin greater incidence.4.ST59 is the most common ST type of Staphylococcus aureus isolated from the nasal cavity in our province.The most common type of MRSA is ST59. |
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