The Effect Of CD200R At The Maternal-fetal Interface On Adverse Pregnancy And The Different Placental Proteomic Analysis With Toxoplasma Gondii Infection

1. The role of CD200R in the abnormal pregnancy outcome of mice induced by T. gondii infection.Objective: To investigate the potential role of CD200R on decidual macrophage in the abnormal pregnant outcomes of mice induced by T. gondii infection. Methods:To explore the potential role of CD200R on decidual macrophage in T. gondii-induced adverse pregnancy outcome, the mice model with T. gondii infection during early pregnancy in vivo were established. The pregnant mice were firstly randomly assigned into infected group and normal control group. Pregnant mice of infected group were injected intraperitoneally with T. gondii tachyzoites on gestation day (GD)8. Dams were executed on GD14 and the pregnant outcomes were recorded. The expression of CD200R and TNF-a on F4/80+ decidual macrophage at maternal-fetal interface (placenta and uterine tissues) were detected by flow cytometry. Human decidual macrophage were preparation and then infected with T. gondii. The expression of CD200R and TNF-α on CD14+ decidual macrophage were detected by flow cytometry. Relationship between the expression of CD200R and the level of TNF-α was analyzed by Spearman’s correlation analysis. Results: Pregnant mice in normal control group showed agile action, while the infected pregnant mice were droopy, shambling and erect fur. Most fetuses and the placenta from the infected group were small in size and had poor blood supplies compared to the normal control group. The abortion rate of infected group was significantly higher, and the weights of placentas and fetuses from infected pregnant mice were significantly less than those from uninfected pregnant mice. Compared with normal control group, the expression of CD200R was decreased while the level of TNF-α was increased on F4/80+ decidual macrophage. The expression of CD200R was decreased while the level of TNF-α was significantly increased on CD14+ decidual macrophage after T. gondii infection. The level of CD200R correlated negatively with the level of TNF-α. Conclusions: The inhibitory receptors of CD200R on decidual macrophage play an important role in the adverse pregnancy outcomes induced by T gondii infection.2. Label-free proteomic analysis of placental proteins during Toxoplasma gondii infectionObjective: To better understand the molecular mechanisms underlying the damage of placenta during T. gondii infection and provide a systematic research to determine molecular mechanisms involved in adverse pregnancy outcomes following T. gondii infection. Methods: Placental proteins extracted from T. gondii-infected and-uninfected pregnant mice were comparatively analyzed using label-free liquid chromatography-tandem mass spectrometry. Quantitative real-time polymerase chain reaction, western blotting, and immunohistochemical staining were used to validate the results of the proteomic analysis. Results: Significant difference was observed in the expression of 58 out of 792 proteins in T. gondii infected placentas. Some placental proteins, such as protease inhibitor, annexins, microtubules, the actin cytoskeleton and several other proteins involved in acetylation, duplication, or secretion were differentially expressed in infected placentas, and these proteins were found to be associated with several different biological processes of pregnancy,particularly with trophoblast invasion, placenta development and host immune response.