Study On The Expression Of 20s Proteasome Alpha 2subunit In Anterior Lens Capsule Of Patients With Age-related Cataract

Objective: According to the different degree of opacity and age-related cataract of different nuclear hardness(Age-related cataract,ARC)were detected and analyzed protein expression in patients with anterior lens capsules of 20 s proteasome subunit alpha2,in order to understand the expression of lens aging and 20 s proteasome alpha 2 subunit protein of different opacity and different kernel hardness the relationship between cataract patients and its significance.Methods: 120 cases of anterior capsular membrane were collected by phacoemulsification or small incision cataract extraction(nuclear in 60 cases,cortical in60 cases).Grouped according to the nucleus hardness and opacity of different,divided into nucleus group(60 eyes of 60 cases)and cortex group(60 eyes of 60 cases)in which nuclear group according to Emery grading standards are divided into two groups,namely group A: young nuclear(I-II)B group,(IV-V: old nuclear degree);the cortex group divided into two groups according to the LOCSII standard,A group: mild opacity(C0-C2),B group(C4-C5): severe haze.Western-Blotting method was used to detect the expression level of 20 s proteasome alpha 2 subunit in the lens capsule of patients with nuclear and cortical ARC.Results: Nuclear cataract,20 s protease in group B alpha 2 subunit protein expression increased compared with A group((0.99 + 0,02)VS(0.58 + 0.08)),the difference was statistically significant(P<0.01);cortical cataract,20 s protease in group B alpha 2 subunit protein expression was enhanced(A group(1.26 + 0,15)VS(0.89 + 0.09)),the difference was statistically significant(P<0.05);also said that nuclear cataract in young than in the old nuclear nuclear 20 s proteasome alpha 2 subunit protein expression and high content of cortical cataract in mild opacity cataract than severe opacity cataract 20 s proteasome alpha 2 subunit protein content is high.Conclusion:he protein expression levels of 20 s protein α2 subunit in the anterior capsule of age-related cataract patients decreased with the degree of lens opacity and the hardness of the nucleus.