| ObjectiveBased on the TCM syndrome differentiation of gastric cancer,study the distribution of TCM syndromes in patients with gastric cancer in proportion,and after the statistics,collected tissue samples of the most common TCM syndromes(deficiency of spleen and stomach type)in patients with gastric cancer,then do iTRAQ and individualized therapeutic molecular detection related research.Methods1.Data of gastric cancer patients were collected by collecting TCM syndrome questionnaire of patients in clinical practice,then,basing on the collected clinical data,the distribution of TCM syndromes and the characteristics of gastric cancer were studied in our hospital.2.Collectting tissue specimens of the most common TCM syndromes of gastric cancer group patients(deficiency of spleen and stomach type),it were divided into gastric cancer group and adjacent normal control group(Abbreviation: control group),then,the isotope relative and absolute quantification technique was used to studied it,and the five objective proteins(FLNA,VASP,CAV1,COL4A2,PRKCA)is verified and validated by using the method of Western blot.3.Individualized therapeutic molecular detection of 6 genes of gastric cancer tissues samples after formaldehyde fixation was done in a few patients,then,the expression of excision repair cross complementing gene 1 and TOPOⅡA m RNA and the corresponding targeted drugs were detected and analyzed.Results1.From more to less,the distribution of TCM Syndromes of gastric cancer is as follows: deficiency cold of spleen and stomach type(42.9%),Syndrome of Qi and blood deficiency(24.1%),blood stasis within the junction(13.4%),stomach yin deficiency type(8.9%),incoordination between the liver and stomach(7.1%),phlegm stagnation type(3.6%).2.The iTRAQ results of gastric cancer group and controlgroup show that:compared with the normal control group,a total of 2770 expressed proteins were found in the gastric cancer group,among them,there were 147 proteins with the up-regulated times of more than 1.2(P<0.05),and there were 159 proteins with the down-regulated times of less than 0.8(P<0.05).Among them,significant differences proteins(P<0.05)involved in the transduction process 41 KEGG signaling pathway,and each signaling pathway has multiple distinct proteins participated in.In the focal adhesion pathway,who have the largest number of differentially expressed proteins,there were14 statistically significant different proteins between it,They are: COL6A3,MYLK,VASP,FLNC,FLNA,ACTN2,PARVA,ACTN1,ITGA5,CAV1,VCL,PRKCA,COL4A2,ITGA1.The five target proteins(FLNA,VASP,CAV1,COL4A2,PRKCA)was validated by Western blot.The Verification results indicated that these five proteins expressiontendency of iTRAQ proteins were approximately similar to their expression of tendency of Western blotting.They are both showing a downward trend,the details are as follows:(1)FLNA,also known as filamin A,the expression of FLNA in gastric cancer group was 0.502 times lower than that in control group.(2)Vasodilator stimulated phosphoprotein(VASP)may be involved in the migration and invasion and / or metastasis of gastric cancer cells,the expression of VASP in gastric cancer group was 0.472 times lower than that in control group.(3)CAV1(Caveolin 1),also known as caveolin-1,the expression of CAV1 in gastric cancer group was 0.604 times lower than that in control group.(4)COL4A2 is a kind of Collagen IV(type 4 collagen,COL IV),the expression of COL4A2 in gastric cancer group was 0.604 times lower than that in control group.(5)PRKCA,also known as protein kinase Ca,the PRKCA of the gastric cancer group was 0.645 times lower than that of the control group.3.Individualized therapeutic molecular detection of 6 genes was performed on formalin fixed surgical specimens of patients with gastric cancer,and two genes were studied.(1)When the excision repair cross complementing gene 1(ERCC1)was low,according to nucleotide excision repair pathway,distinguish and Identify the damage site on the DNA chain for shear,transcription,coding,etc.At this time,the sensitivity of platinum drugs to tumor cells is higher,the application of platinum containing chemotherapy can improve the clinical efficacy of chemotherapy.(2)According to recommendations for genetic testing reports provided by the cooperative unit and related literature reports,Etoposide antitumor effect by inhibiting the activity of TOPOⅡA,When the TOPO II A m RNA expression levels are high,it is higher sensitivity to etoposide,in clinical,for these patients,the choice of chemotherapy regimens containing etoposide chemotherapy drugs is appropriate.Conclusion1.In the Classification of the traditional Chinese Medicine types of gastric cancer,the most common type of syndrome is deficiency cold of spleen and stomach.Treatment can choose Fuzi Lizhong decoction,or bu Zhong Yi Qi Tang et al.2.In the iTRAQ test of the gastric cancer group and control group,a total of 2770 proteins were found,and these differentially expressed proteins are involved in 41 signal transduction pathways.Then,the five target proteins of the Focal adhesion pathway were selected for discussion,the expression of FLNA,VASP,CAV1,COL4A2,PRKCA in gastric cancer was significantly lower than the control group.The five protein expression tendency in Western Blot and iTRAQ is similarly same.Presumably,when extracellular COL4A2 expression is down regulated,according to signal transduction,the expression of CAV1 on the cell membrane was also down regulated,then,affected by them,the expression of FLNA,VASP and PRKCA in the cells was also decreased.3.Individualized therapeutic molecular detection of 6 genes was performed on a small number of gastric cancer patients,it show that:when the expression of the excision repair cross complementing gene 1(ERCC1)of gastric cancer was low,considering its high sensitivity toplatinum drugs,it is possible to select the chemotherapy regimen containing platinum drugs;when the expression of the TOPO II A m RNA expression levels are high,considering its high sensitivity to to etoposide,the choice of chemotherapy regimens containing etoposide is appropriate. |
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