KDM2B Regulates EZH2 Expression In Epithelial Ovarian Cancer And Influences Cell Proliferation And Migration Ability

ObjectiveTo investigate expression of histone demethylase KDM2B and histone methyltransferases EZH2 in normal ovarian tissues,benign tumor,borderline tumor and ovarian carcinoma,analyzing the correlation between both of them with clinical pathologic characteristics of ovarian carcinoma,confirming the relationship between KDM2B and EZH2 expression.Moreover,our studies were aimed at exploring the influence of KDM2B knockdown on biological characteristics of epithelial ovarian cancer and demonstrating whether EZH2 expression was regulated by KDM2B in ovarian cancer cells.MethodsImmunohistochemistry and real-time PCR were used for detecting KDM2B and EZH2 expression in normal ovarian tissue,benign tumor,borderline tumor and ovarian cancer respectively.KDM2B short hairpin RNA(shRNA)expression lentivirus was constructed and subsequently used for ovarian cancer cell line A2780 and SKOV3 infection.The mRNA and protein level of EZH2 was analyzed by real-time PCR and Western blot after obtained shKDM2B-stable expressed ovarian cancer cells.The effect of KDM2B knockdown on cell proliferation was detected by cell counting kit-8(CCK-8 assay),wound healing assay and Transwell chambers were used for detecting cell migration ability.ResultsKDM2B and EZH2 were high expressed in ovarian carcinoma,and both of them were associated with tumor histological type,tumor grade,FIGO stage and lymphatic metastasis.There was a positive correlation between KDM2B and EZH2 in ovarian cancer tissues.KDM2B expression in A2780 and SKOV3 cells were successfully inhibited by KDM2B targeted shRNA,which then induced EZH2 downregulation and declined the proliferation and migration of ovarian cancer cells.ConclusionsKDM2B may be involved in the development and progression of epithelial ovarian cancer through regulating EZH2 expression,and it can be regarded as a potential target for ovarian cancer therapy.