Study On The Intervention Effect Of Arsenic Trioxide On The Expression Of EZH2 And MRNA In Acute Myeloid Leukemia U937 Cells

Objects:This paper analyzes how As₂O₃,the main component in arsenic,will influence on the expression of EZH2 mRNA in u937 cell group in Acute Myelogenous Leukemia（AML）,thus to provide new methods and ideas for the research on pathogenesis and curing of leukemia from biology functions of EZH2 genes.Methods:Adopt CCK-8 method to detect how different concentrations of As₂O₃ will inhibit the proliferation of u937 cell line;under the inversed microscope,observe and photograph the morphology of regular cells and u937 cells cultivated under different As₂O₃ concentrations;use Real-Time PCR method to detect the expression of EZH2 mRNA in u937 cells before and after medication.Results:Cultivated u937 cells with different concentrations of As₂O₃,which provided different degrees of inhibitions for the proliferation of u937 cell line,and found that the inhabitation rate increased with concentration.Separately chose 25nmol/L,50nmol/L,100nmol/L and 200nmol/L concentrations of-As₂O₃ in which u937 cell lines were cultivated for 24h,and found that the expression of EZH2 mRNA deceases under different degree.Comparing each experimental group with control group and within each experimental group,the expression differences of EZH2 was found to be statistical（P<0.05）.Therefore,As₂O₃ can enhance the apoptosis of u937 cells,and the degree depends on time and dosage.Conclusion:As₂O₃ can inhibit the proliferation of u937 cell lines and induce the apoptosis,lowering the expression level of EZH2 mRNA.