| ObjectiveTo explore the effect of behavioral development and cognition in the offspring rats after exposure to 1800MHz and WiFi radio-frequency field.Method24 Wistar Female rats and 12 Male rats at age of 7 week-old were randomly divided into four groups(6 rats in each) after one week for environmental adaptation and successful mating.Rats in the exposure groups were respectively and continuously exposure, between 0 days of pregnancy and 20 days of pregnancy (12 hours/day) to 1800MHz microwave with power density of 1.0 mW/cm2 or 1800MHz and WiFi radio-frequency field. Simultaneously,two sham-controls were carried out respectively.Pregnant rats during exposure in each group were all free drinking and eating, and the environmental background conditions (including temperature, humidity, background noise, aeration, lighting, etc) were stable. After the completion of exposure the indicators of physiological development and behavioral development were observed and measured in offspring rats. When the offspring rats were 3 week and 7week old,Morris water maze were conducted to observe the behavior change. Subsequently,high performance liquid chromatography were used to detect amino acid neurotransmitters expression of glusate, glycine, y-aminobutyric acid, aspartic acid in rat hippocampus.Results1. Development index: (1) Physiological development indicators showed a significant trend of weight lossing and eyes opening time prolonged in two experimental groups compared to control groups (p<0.05).(2)Behavioral development indicator showed a significant difference in forelimb hanging test between two experimental groups and each control group (p<0.05).2. Morris water maze: (1) Morris water maze in 3-week-old offspring rats:Visible platform test showed, exposed to 1800MHz electromagnetic radiation , there was a significantly prolonged of male rats escape latency in experiment groups compared with control groups in second day(p<0.05). And it was significantly prolonged of female rats in experiment groups compared with control groups in both first day and second day(p<0.05). Hidden platform showed, there was a significantly prolonged of male rats escape latency in experiment groups compared with control groups from first day to fourth day(p<0.05). While it was significantly prolonged of female rats in experiment groups compared with control groups from first day to trird day (p<0.05). Visible platform test showed, exposed to 1800MHz and WiFi radio-frequency field , there was a significantly prolonged of female rats escape latency in experiment groups compared with control groups in first day(p<0.05).And the escape latency of both male and female rats in experiment groups presented a significantly prolonged than control groups from first day to fourth day exposed to 1800MHz and WiFi radio-frequency field (p<0.05).(2) Morris water maze in 7-week-old offspring rats: Visible platform test showed, exposed to 1800MHz electromagnetic radiation, there was a significantly prolonged of male rats escape latency in experiment groups compared with control groups in second day(p<0.05). Hidden platform showed, there was a significantly prolonged of male rats escape latency in experiment groups compared with control groups in second day and third day(p<0.05). While it was significantly shortened of female rats in experiment groups compared with control groups in first day (p<0.05). Spatial probe test showed, the average number of female rats passing the platform in experiment groups more than control groups, and time percentage of platform quadrant was higher in experiment groups. When rats were exposed to 1800MHz and WiFi radio-frequency field, Hidden platform showed that escape latency was significantly shortened in experiment groups compared with control groups among male offspring rats in first day, third day and fourth day. And it was significantly shortened of female rats in experiment groups compared with control groups in fourth day(p<0.05). Spatial probe test showed the time percentage of platform quadrant in experiment groups was higher than control groups.3. High performance liquid chromatography test indicated that the expression of Aspartic acid in 3-week-old offspring rats hippocampus presented up-regulated in experiment groups compared to control groups. When rats were exposed to 1800MHz and WiFi radio-frequency field, Glutamic acid in 7-week-old offspring rats hippocampus presented down-regulated in experiment groups compared to control groups.And the expression of Aspartic acid in 3-week-old rats was significant higher than 7-week-old rats.Conclusion1. There was an effect on body weight and eyes opening time of offspring rats exposed to 1800MHz and WiFi radio-frequency field; while the impact on forelimb hanging test needed to be further verification.2. There was an effect on spatial learning ability of 3-week-old offspring rats exposed to 1800MHz and WiFi radio-frequency field, while the effect on 7-week-old offspring rats needed to be further validated.3. High performance liquid chromatography test indicated that the expression of Aspartic acid in 3-week-old offspring rats hippocampus presented up-regulated in experiment groups compared to control groups. When rats were exposed to 1800MHz and WiFi radio-frequency field, Glutamic acid in 7-week-old offspring rats hippocampus presented down-regulated in experiment groups compared to control groups.4.The effects of WiFi on offspring rats learning and memory ability remained unclear. |
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