The Expression Level Of OLC1 Protein In Tissues Of Patients With NSCLC And Its Relationship With Prognosis

Background and objective:Overexpressed in lung cancer 1(OLC1)is a new gene related to lung cancer,which was proved to be overexpressed in lung Squamous cell cancer(SCC),lung Adenocarcinoma(ADC)and Small cell lung cancer(SCLC)in previous research.OLCl protein in plasma of lung cancer patients is significantly higher than that of normal people,and could be used as a candidate maker for the diagnosis of lung cancer.OLC1 was also overexpressed in patients with Esophageal Squamous Cell Carcinoma,Ovarian cancer,Breast cancer and colorectal cancer,besides,was related with poor prognosis of these patients.But the relationship between the OLC1 expression level and the prognosis of lung cancer patients is still unknown.The aim of this research was to detect the expression level of OLC1 in the tumor tissues and adjacent normal lung tissues of NSCLC patients,and explore its correlation with the prognosis of patients.Methods Immunohistochemistry was performed to detect the expression of OLC1 protein in lung cancer tissues and adjacent normal lung tissues of 75 SCC and 90 ADC patients on two tissue microarrays and another 33 permanent paraffin sections of SCC,and the relationship between the expression level of OLC1 with clinical parameters and prognosis was analyzed.Results The overexpression rate in lung cancer tissues of OLC1 with ADC(87.5%)was significantly higher than that of SCC(68.6%,P=0.005).However,the overexpression of OLC1 in lung cancer tissues has no relationship with the prognosis of Adenocarcinoma,the overexpression of OLC1 in Squamous cell carcinoma was associated with poor prognosis,the univariate Kaplan-Meier survival analysis revealed that patients with OLC1 overexpressed had a poorer prognosis(HR=2.649,95%CI 1.039-6.754,P=0.041).The multivariate Cox regression analysis showed that the survival time between the two group didn’t mean a significant difference(HR=2.048,95%CI 1.00-4.19,P=0.05).The expression of OLC1 was more common in patients olderthan 60 years(P=0.002),when OLC1 overexpression combined with age as a risk factor,patients tended to have a significant poor prognosis(HR=5.125,95%CI 1.756-14.96,P=0.003).Conclusions The overexpression rate of OLC1 in Adenocarcinoma was significantly higher than that of Squamous Cell Carcinoma,but the overexpression of OLC1 in tumor tissues had no correlation with the prognosis of ADC patients.The overexpression of OLC1 was more common in patients with SCC older than 60 years(P=0.002),and it might affect the prognosis of SCC patients together with age.The overexpression of OLC1 in lung cancer tissues was not an independent risk factor for SCC patients.