The Expression And Clinical Significance Of Rad9,Rad1,Hus1 Gene In Human Colorectal Cancer

Objective:Dtecting the expression levels of Rad9,Rad1,Hus1 gene,combined with the pathologic differentiation degre,staging,lymph node metastasis of tumor,discussing whether Rad9,Rad1,Hus1 gene has a certain relationship between the colorectal cancer and providing a basis for that whether Rad9,Rad1,Hus1 gene can be an important biomarker for colorectal cancer,which can provides a basis for early diagnosis,early treatment,prognosis of colorectal cancer.Method:We selected colorectal cancer tissue and matched para-cancer tissues from 35 patients with colorectal cancer.Rad9,Rad1,Hus1 mRNA expression levelsof colorectal cancer tissue and matched para-cancer tissues are tested by using quantitative reverse transcription—PCR andanalyzed whether they are different,whether the expression differences are related to TNM stage of the tumor,the degree of pathological differentiation,parts,gender,age,etc.The results of this study are statistically analyzed by Spss17.0 software,P < 0.05 for the difference is statistically significant.Results:1.There are 25(71.43%)colorectal cancer tissues which have lowerexpression levels of Rad9 mRNA than that in para-cancer tissues.The expression levels of Rad9 mRNA in colorectal cancer tissues(2.98±5.14)lower than that in para-cancer tissues(7.11±16.82),the differences are considered as statistically significant(Z=-2.064,P=0.039).2.There are 13(92.85%)low-grade tumors which have lowerexpression levels of Rad9 mRNA and 9(64.29%)middle-grade tumors which have lowerexpression levels of Rad9 mRNA,there are 3(42.86%)high-grade tumors which have lowerexpression levels of Rad9 mRNA,the differencesare considered asstatistically significant(P=0.033).3.There are 16(88.89%)colorectal cancer tissues with lymph node metastasis which have lower expression levels of Rad9 mRNA and 9(52.94%)colorectal cancer tissues without lymph node metastasis which have lower expression levels of Rad9 mRNA,the differencesare considered as statistically significant(P=0.027).4.There are respectively 1(25.00%),8(61.54%),10(83.33%),6(100%)colorectal cancer tissues which have lower expression levels of Rad9 mRNAinⅠ,Ⅱ,Ⅲ,Ⅳ of TNM stages of colorectal cancer tissuesthe differences are considered as statistically significant(P=0.042).5.The expression differences of Rad9 mRNA are not statistically significant in different ages,genders,and locations(P>0.05).6.There are 16(45.71%l)colorectal cancer tissues which have lowerexpression levels of Rad1 mRNA than that in para-cancer tissues.The expression levels of Rad1 mRNA in colorectal cancer tissues(2.01±4.62)lower than that in para-cancer tissues(2.61±6.49),the differences are not considered as statistically significant(Z=-0.402,P=0.712)7.There are 14(40.00%)colorectal cancer tissues which have lowerexpression levels of Hus1 mRNA than that in para-cancer tissues.The expression levels of Hus1 mRNA in colorectal cancer tissues(4.04±7.51)higher than that in para-cancer tissues(3.36±8.30),the differences are not considered as statistically significant(Z=-0.292,P=0.784).Conclusion:1.The expression levels of Rad9 mRNA in colorectal cancer tissueslower than that in para-cancer tissues,which suggested that expression levels ofthe Rad9 may be related to colorectal cancer.2.The lower pathological differentiation,the later clinical stage and the metastatic lymphonode,the higher rate was the lower expression rate of Rad9 mRNA in colorectal cancer,which maybe closely related to aggressiveness and transferability of colorectal cancer.3.The expression levels of Rad9 mRNA is not considered to be related to the gender and age of patients,tumor location.4.The expression levels of Rad1,Hus1 mRNA is not considered to be related to colorectal cancer.