Association Of The SLC39A8 Rs971752 Snpand Blood Lipid Levels In The Jing And Han Populations

Background: A large number of epidemiological investigation confirmed that dyslipidemia is relatived to the occurrence and development of cardiovascular disease and it is affected by the interaction of genetic variations and environmental factors.Recently,genome wide association studies(GWASs)have reported that the solute carrier family 39 member 8 gene associated with serum lipid levels.However,the effect has not been validated previously in china.Therefore,the present study aimed to explore the association of the SLC39A8 rs971752 SNP and some environmental factors with blood lipid levels in the Jing and Han populations.Methods:This study included 741 unrelated subjects of Jing nationality and 742 unrelated participants of Han nationality who live in Dongxing City,Guangxi Zhuang Autonomous Region,People’s Republic of China.Randomly select them from ehemalig stratified(sex and age)randomized samples.Useinternationally standardized methods to get the epidemiological data.The data were collected using standardized questionnaires including the features of demography,lifestyle factors,socioeconomic status,cardiovascular disease medication history,past medical history,family history.In the physical examination,several parameters such as height,weight,and waist circumference were measured.A venous blood sample was obtained from all participants in a fasting state and used to measure serum lipid levels and extract deoxyribonucleic acid(DNA).We use the phenol-chloroform method to isolate genomic DNA.Genotyping of the SLC39A8 rs971752 SNP was detected by polymerase chain reaction(PCR)and restriction fragment length polymorphism(RFLP).Afterwards,the genotypes detected by the PCR-RFLP were also confirmed by direct sequencing.Results: The levels of ApoA1,the ratio of ApoA1 to ApoB and the percentages of alcohol consumption were higher in Han than in Jing(P < 0.05),whereas the values of body weight,BMI,waist circumference,blood glucose,TC,TG were lower in Han than in Jing(P < 0.001 for each).There were no significant differences in the gender ratio,age structure,the percentage of cigarette smoking,and pulse pressure,diastolic blood pressure,systolic blood pressure,body height,serum ApoB,LDL-C and HDL-C levels between the two ethnic groups(P > 0.05 for all).The distribution of genotypes in both Jing and Han was conformed with the Hardy-Weinberg equilibrium(HWE,P > 0.05 for each).The frequencies of AA,GG and AG genotypes were 82.2%,1.3% and16.5% in Jing,and 75.6%,1.1% and 23.3% in Han(P < 0.01);separately.The SLC39A8 rs971752 G allele frequency was higher in Han than in Jing(12.7% vs.9.6%,P < 0.01).There was no significantly statistical difference in the genotypic and allelic frequencies between males and females in the both ethnicgroups(P?>?0.05 for all).The G allele carriers had higher TG levels,and lower serum TC,HDL-C and LDL-C levels than the G allele non-carriers.Subgroup analyses showed that serum levels of TC,HDL-C and LDL-C in Jing males and TG levels in Jing females were different between the AA and AG/GG genotypes(P < 0.05 for all),the G allele carriers had higher serum TG levels in Jing females,and lower serum TC,LDL-C and HDL-C levels in Jing males than the G allele non-carriers.Multiple linear regression analysis showed that serum TC levels in the combined population of Han and Jing;and TC,HDL-C,LDL-C levels in Jing but not in Han were correlated with the genotypes of the SLC39A8rs971752 SNP(P < 0.05).When the correlation of serum lipid parameters and the genotypes was completed according to sex subgroups,we discovered that serum TC,HDL-C and LDL-C levels in Jing males but not in females were correlated with the genotypes(P < 0.05).Boold lipid parameters were also relevant to some environmental factors such as height,weight,age,BMI,waist circumference,cigarette smoking,alcohol consumption,blood glucose and blood pressure in both ethnic groups(P < 0.05-0.001).Conclusions: the present research showed that the genotypic and allelic frequencies of the SLC39A8 rs971752 SNP were different between the Jing and Han populations.The association of the SLC39A8 rs971752 SNP and serum lipid levels was found in the Jing but not in the Han populations,and gender difference is existed in the Jing populations.These findings suggest that the association between the SLC39A8 rs971752 SNP and serum lipid levels might have ethnic-and/or sex-specificity.