| Objective:This project is to reveal the biological behavior of the regulation of Chuanglingye on the inflammatory response of breast cancer surgery to affect the breast cancer cells,and to explore the operation effect of Chinese medicine in better treating breast cancer.Method:30 breast cancer patients were randomly divided into treatment group and control group.The treatment group received basic treatment,and rinsed the wound with Chuanglingye after routine irrigation during operation,20ml each time,sucked by drainage tube after 10 minutes,while the control group only received basic treatment.The levels of axillary drainage fluid,IL-2,IL-4,IL-6,TNF-α,and white blood cell were measured on the 1st,4th and 7th day after breast cancer operation.The part of cell experiments were determined by MTT for the 0.5%and 1%drainage effect of culture MDA-MB-231 cells and MCF-7 cells on its proliferation rate.Result:1.The local inflammation of the treatment group was significantly improved,and the score was significantly lower than the control group,(experimental group D1 scored 8.21±1.40,control group D1 scored 9.09 ± 2.02,p=0.186;experimental group D4 scored 3.26±1.65,control group D4 scored 4.55±1.88,p=0.07;experimental group D7 scored 2.33±1.33,control group D7 scored 4.45 ± 2.31,and on the 7th day after operation,there was a significant statistical difference,p = 0.004)2.The levels of IL-4,IL-6 and TNF-a in the drainage fluid on the 7th day after operation were significantly lower than those in the control group.IL-4 group(experimental group D1 value 2.08 ±1.32,control group D1 value 3.37 ± 1.71,p = 0.034;experimental group D4 value 1.90 ± 1.44,control group D4 value 4.04 ± 2.85,p = 0.025;experimental group D7 value 1.29±0.96,P<0.05);IL-6 group(experimental group D1 value 52.66 ± 8.13,control group D1 value 55.54 ± 5.70,p=0.002;experimental group D4 value 48.33±4.78,control group D4 value 53.38±6.21,p=0.001;experimental group D7 value 47.62± 8.78,control group D7 value 54.31±6.42,p=0.001);TNF-α group(experimental group D1 value 37.98±7.02,control group D1 value 67.71±36.88,p=0.008;experimental group D4 value 36.85±9.62,control group D4 value 48.75± 22.51,p = 0.001;experimental group D7 value 36.17 ± 9.26,control group D7 value 56.58±23.80,p=0.007).The level of L-2 was significantly higher than that of the control group.(experimental group D1 value 7.57±1.61,control group D1 value 7.35±2.01,p=0.977;experimental group D4 value 7.57±1.3,control group D4 value 6.54 ±1.18,p＝0.042;experimental group D7 value 7.61±1.46,control group D7 value 6.40±0.92,p＝0.007).3.Chuanglingye can significantly reduce the serum levels of CRP.The level of CRP in the experimental group was significantly lower than that in the control group on the 7th day after operation(experimental group preoperative value 3.62±1.43,control group preoperative value 3.66±1.34,p=0.129;experimental group D1 value 13.51±7.48,control group D1 value 12.16±9.85,p=0.719;experimental group D4 value 9.15± 6.54,control group D4 value 10.76±6.61,p=0.616;experimental group D7 value 4.50±2.37,control group D7 value 9.03±4.29,p=0.007);Chuanglingye lead to a lower trend on the serum white blood cell level,there is no statistical difference(experimental group preoperative value 4.19±0.34,control group preoperative value 4.42±0.63,p=0.310;experimental group D1 value 9.49±3.65,control group D1 value 9.69± 2.89,p = 0.880;experimental group D4 value 5.75±1.28,control group D4 value 6.25±2.04,p=0.488;experimental group D7 value 5.56± 0.97,control group D7 value 6.25 ± 1.51,p = 0.242).4.Chuanglingye’s intervention on MDA-MB-231 cell proliferation has statistical differences on the lst,4th,and 7th days.0.5%concentration of 24 hours group(experimental group D1 value 115.44±25.89,control group D1 value 127.79±14.25,p=0.027;experimental group D4 value 112.11±26.13,control group D4 value 130.40±16.63,p=0.001;experimental group D7 value 108.55±23.99,control group D7 value 131.12±13.54,p<0.001);1.0%concentration of 24 hours group(experimental group D1 value 115.68 ± 20.19,control group D1 value 115.44±25.89,p=0.001;experimental group D4 value 110.45± 22.57,control group D4 value 119.48±13.54,p<0.001;experimental group D7 value 104.75±20.43,control group D7 value 122.33± 16.15,p<0.001);0.5%concentration of 48 hours group(experimental group D1 value 135.96 ± 40.36,control group D1 value 174.81 ± 11.23,p＝0.001;experimental group D4 value 142.03±22.15,control group D4 value 172.69±15.33,p<0.001;experimental group D7 value 120.79± 42.64,control group D7 value 172.69 ±15.33,p<0.001);1.0%concentration of 48 hours group(experimental group D1 value 140.52 ±30.05,control group D1 value 173.75± 10.62,p = 0.031;experimental group D4 value 139.15±17.45,control group D4 value 169.65± 2.28,p = 0.034;experimental group D7 value 120.94 ± 27.16,control group D7 value 168.89 ±11.53,p=0.018).5.5.Chuanglingye’s intervention on MCF-7 cell proliferation has statistical differences on the 1st,4th,and 7th days.0.5%concentration of 24 hours group(experimental group D1 value 103.73±10.37,control group D1 value 119.92±21.99,p=0.003;experimental group D4 value 104.98± 6.22,control group D4 value 117.43±17.01,p=0.003;experimental group D7 value 103.32±9.13,control group D7 value 119.92±19.09,p = 0.001);1.0%concentration of 24 hours group(experimental group D1 value 105.39 ± 9.96,control group D1 value 133.61 ±21.99,p<0.001;experimental group D4 value 103.32± 9.54,control group D4 value 125.73 ±13.28,p<0.001;experimental group D7 value 103.32±14.94,control group D7 value 119.92±19.09,p<0.001);0.5%concentration of 48 hours group(experimental group D1 value 108.96±22.64,control group D1 value 121.38 ± 15.13,p = 0.031;experimental group D4 value 104.48 ±26.62,control group D4 value 120.89 ± 12.17,p = 0.010;experimental group D7 value 97.26±29.10,control group D7 value 124.34 ± 14.16,p = 0.008);1.0%concentration of 48 hours group(experimental group D1 value 118.41 ± 19.40,control group D1 value 172.04 ± 40.79,p<0.001;experimental group D4 value 114.43 ± 20.40,control group D4 value 169.74 ± 29.93,p<0.001;experimental group D7 value 109.20 ± 21.39,control group D7 value 169.08 ± 46.05,p<0.001).6.Chuanglingye lead to a lower trend on the drainage volume,there is no statistical difference(experimental group D1 value 76.88 ± 35.26,control group D1 value 84.09± 40.27,p=0.639;experimental group D4 value 31.31 ± 20.16,control group D4 value 38.18 ± 25.16,p =0.478;experimental group D7 value 17.20 ± 13.78,control group D7 value 23.46 ± 21.99,p ＝0.401).Conclusion:The treatment of breast cancer surgery can significantly improve the inflammatory symptoms of breast cancer surgery,regulate the inflammatory factors of breast cancer patients and drainage fluid stimulating tumor growth-related inflammatory factors,improve the microenvironment of breast cancer postoperative wound,inhibit breast cancer cell growth,and can play a dual role in promoting the body repair and fighting against tumor. |
PDF Full Text Request