Study On The Mechanism Of Pluronic F127 Modified Graphene Oxide Loading Doxorubicin To Glioma

Objective:Study on the effect and its molecular mechanism of pluronic F127 modified graphene oxide loading doxorubicin to glioma and provide more evidence for the signal pathway of tumor targeting therapy.Methods:(1)human glioma cell line U251 cells: were resuscitated,cultured and stored;(2)compared the effects of PG,PF127-GO,DOX and PF127-GO on apoptosis of U251 cells: the experiment was divided into 4 groups: control group,PF127-GO group,DOX group and PF127-GO-DOX group,and the apoptosis rate was detected by flow cytometry;(3)extracted U251 cell cytoplasmic protein,and studied the ptotein expression level of extracellular signal-regulated kinases 1/2,c-Jun amino(N)-terminal kinases,Caspase-3 and P53 with Western-blotting;Results:(1)PF127-GO-DOX and DOX significantly promoted the apoptosis of glioma U251 cells;(2)PF127-GO-DOX could up-regulate the protein expression of P53,JNK,Caspase and down-regulate the expression of ERK1/2.Conclusion:(1)PF127-GO-DOX had a stronger effect on promoting the apoptosis of U251 cells,and it was superior to the single DOX;(2)PF127-GO-DOX could activate JNK and P53 pathway,andinhibit ERK1/2 pathway to induce tumor cell apoptosis,PF127-GO-DOX play an inporant role of glioma therapy.