Feasibility Analysis Of Flurescence Virus Biosensor Based On CNPS

ObjectiveTo establish DNA fluorescence biosensors based on CNPs for detection of EV71 and analyze feasibility,sensitivity and influence factor of it.MethodsCNPs was prepared from the candle ashes and detect its characterization.To analyze feasibility of biosensors,EV71 was detected with or without CNPs and change of fluorescence intensity was recorded.To analyze sensitivity of biosensors,complete complementary sequence,mismatched sequence and uncomplementary sequence were detected.To analyze CNPs concentration effects on biological sensors,sequence was detected with a series of concentration.ResultsCNPs have ability to adsorb ssDNA and quench fluorescence,it could be used for detection of DNA.And this biosensors have high sensitivity and repeatability,it could differentiate single-base mismatch.Meanwhile,it was positively associated with the degree of concentration and quenching,but high concentrations could reduce the efficiency of hybridization and degree of fluorescence restoration.ConclusionThe DNA fluorescence biosensors based on CNPs have been established for detection of DNA and monitoring the interaction of the other biological molecules as a probe.CNPs/DNA system is easy to detect,and have better detection sensitivity,selectivity and lower detection limitation.