| Severe preeclampsia(Severe preeclampsia s PE)is a multisystem disease characterized by high blood pressure and damage to the system,which is one of the main causes of maternal and neonatal mortality increases.s PE can be characterized as EOSPE(<34 weeks gestation)or LOSPE(≥34 weeks gestation).The aetiology and pathogenesis of s PE are not fully understood,but most scholars believe that the placental pathogenesis involves shallow implantation and shallow trophoblast invasion.Lysine(K)-specific demethylase 5C(KDM5C),may act as an oncogene.Furthermore,KDM5 C promotes cancer cell proliferation..Bone morphogenetic proteins(BMPs)are members of the TGF-beta superfamily of growth factors.BMPs are known for their roles in the regulation of osteogenesis and developmental processes.BMPRII,binds ligands within BMP-7.Ectopic expression of KDM5 C in HCC cells promoted cell migration,invasion and epithelial-mesenchymal transition via the inactivation of BMP-7.There are considerable similarities to malignant tumour cell infiltration of surrounding tissues and distant metastasis.KDM5C-BMP7 signaling pathway is involved in the pathogenesis of severe preeclampsia has not been reported.Objective The study by detecting KDM5 C,BMP7 and its receptor BMPRII expression in placenta.preliminary Discussion on KDM5C-BMP7 signal transduction mechanisms in the pathogenesis of severe preeclampsia.Materials and methods 1.Subject According to the People’s Medical Publishing House published the eighth edition of the Obstetrics and Gynecology.From September 2014-January 2016 in the Third Affiliated Hospital of Zhengzhou University Hospital clinical diagnosis in obstetrics For early onset severe preeclampsia and late onset severe preeclampsia patients with 30 cases.Select the same period under anesthesia with epidural block anesthesia 30 cases of patients with caesarean delivery.The ethics committee approval and informed consent of patients and their families.Participants included for singleton births.And eliminate chronic high blood pressure,diabetes,Heart disease,immune system disorders,polycystic ovary syndrome and other complications.2.Methods In the sterile operation were collected within 15 minutes after the delivery of the placenta,pay attention to avoid the calcification,necrosis,respectively,in the middle of the placenta and the surrounding position to take five full-thickness tissue,the size of about 2cm × 2cm × 1cm,Brine rinses the blood on the tissue block and puts it in formaldehyde for immunohistochemistry.The remainder is enclosed in a sterile cryopreservation tube and immediately placed in a liquid nitrogen tank and transferred to a refrigerator at-80 ° C for storage.The m RNA and protein expressions of KDM5 C,BMP7 and BMPRII were detected by immunohistochemical method.Real-time quantitative PCR and Western blot were used to detect the m RNA and protein expression of KDM5 C,BMP7 and BMPRII in the three placenta tissues.The role of KDM5C-BMP7 pathway in the pathogenesis of severe preeclampsia.3 statistics analysis SPSS 21.0 statistical software was used for statistical processing.Quantitative data results are expressed as mean ± SD.Qualitative data were compared using chi-square test.(LSD)method,which does not accord with the normal distribution or the variance homogeneity with nonparametric Kruskal-Wallis test.The results show that the number of independent samples is normal and the variance is the same.To α = 0.5 for the test level.Results 1 Comparison of general clinical data of three groups of pregnant women 1.1 No statistically significant differences in clinical data.There was no significant difference in the average age of pregnant women,the increase of body weight during pregnancy,the fasting blood glucose during pregnancy and the number of gestational times(P> 0.05).1.2 There were statistically significant differences in clinical data.The number of days of gestation(days),systolic blood pressure,diastolic blood pressure,neonatal birth weight,birth score of 1 minute,5-minute A’s score,and early-onset severe preeclampsia were less than those of late-onset The number of days of gestation in severe preeclampsia group and late-onset severe preeclampsia was lower than that in the normal control group.The early-onset severe preeclampsia group had a higher blood pressure and a higher incidence of severe preeclampsia in preeclampsia group.The scores were significantly lower in the early hair type,the difference between the three groups were statistically significant.This indicates that the smaller the gestational age,the more severe the complication and complication of mother and child,the worse the prognosis(P <0.05).2 Expression of KDM5 C,BMP7 and BMPRII m RNA in three placenta tissues.2.1 Expression of KDM5 C m RNA in three groups of placenta.The expression of KDM5 C m RNA was 2.19 ± 0.34 in the normal control group,1.05 ± 0.81 in the late-onset group and 0.82 ± 0.86 in the early-onset group.The difference between the three groups was Statistically significant(P <0.05).2.2 Expression of BMP7 m RNA in three groups of placenta The relative expression of BMP7 m RNA was 0.54 ± 0.25 in the normal control groupand 0.82 ± 0.12 in the late-onset group.The relative expression of BMP7 m RNA was 1.35 ± 0.22 in the early-onset group,There was significant difference between the three groups(P <0.05).2.3 Expression of BMPRII m RNA in three groups of placenta.The relative expression of BMPRII m RNA in the normal control group was 0.39 ± 0.10 and 0.55 ± 0.11 in the late-onset group,and the relative expression was 1.02 ± 0.13 in the early-onset group 。The difference between the three groups was Statistically significant(P <0.05).3 Expression of KDM5 C,BMP-7 and BMPRII proteins in three groups of placenta.The expression of KDM5 C,BMP7 and BMPRII protein was detected in the three groups of placental tissues.The KDM5 C protein was mainly expressed in the nucleus and vascular endothelial cells of placental syncytiotrophoblast and trophoblast cells,and was positive in the early stage of severe preeclampsia(P <0.05).The expression of BMP7 protein was mainly expressed in the placenta accreta(P <0.05),and the expression of BMP7 protein was significantly higher than that of the normal group(P <0.05).The expression of BMP7 protein was significantly higher than that of the normal preeclampsia group The BMPRII protein was mainly expressed in placental syncytiotrophoblast and trophoblast cells on the cytoplasm of trophoblast cells and trophoblast cells.Compared with the normal control group,the BMP7 and BMPRII proteins were positive in the late-onset severe preeclampsia group(P <0.05),but there was significant difference between the three groups(P <0.05),but the difference was statistically significant(P <0.05).4 KDM5 C,BMP-7 and BMPRII protein expression in the placenta.4.1 Relative expression of KDM5 C protein in three groups of placenta.The results of Western blot showed that the relative expression of KDM5 C protein was 0.32 ± 0.45,0.21 ± 0.35 and 0.11 ± 0.05 in normal control group,late onset severe preeclampsia group and early onset severe preeclampsia group.The relative expression of KDM5 C protein was statistically significant(P <0.05).4.2 Relative expression of BMP7 protein in three groups of placenta.The relative expression of BMP7 protein in normal control group,early-onset severe preeclampsia group and late-onset severe preeclampsia group was 0.05 ± 0.07,0.10 ± 0.09,0.11 ± 0.25,the relative expression of BMP7 protein in three groups of placenta The difference was statistically significant(P <0.05).4.3 Relative expression of BMPRII protein in three groups of placenta.The relative expression of BMPRII protein in the normal control group,early-onset severe preeclampsia group and late-onset severe preeclampsia group were 0.03 ± 0.02,0.08 ± 0.19,0.16 ± 0.15,the relative expression of KDM5 C protein in the three groups of placenta The difference was statistically significant(P <0.05).Conclusion In abnormal placental tissue of severe preeclampsia,abnormal expression of KDM5 C,BMP7 and BMPRII may be involved in the development of severe preeclampsia,and this effect is more obvious in the early stage of severe preeclampsia.Which will help us to further understand and understand the causes of severe preeclampsia,to find a new molecular marker to provide a theoretical basis. |
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