Effects Of Propionibacterium Freudennreichii On TLR2/NFκB Signaling Pathway In Mouse Colon Cancer Cells

Colon cancer is the third most difficult cancer in the world,accounting for 10.0%of the new cancer cases registered of about 14.1 million new cases in 2012.Although the current clinical application of the treatment of colon cancer for the wide range of drugs,but these drugs are not perfect,with different effects of side effects,the future for new,non-toxic,no side effects of a comprehensive research and development of drugs,the colon cancer for the prevention and treatment is always the focus of research.Probiotics can regulate expression of inflammatory factors and inflammatory cytokines in intestinal,The role of probiotics in intestinal immunity plays an more important role.Recent studies have shown that the use of probiotics can slow the growth of the tumor,regulate the host immune response and the diversity of intestinal flora,and rebuild the healthy intestine.Propionibacterium freudennreichii(P.freudennreichii)as an effective probiotic in the digestive tract to prevent cancer,could produce short-chain fatty acids acting on the cell mitochondria,and ultimately induce colorectal cancer cell apoptosis;Fermented milk of P.freudennreichii can cause apoptosis of human gastric cancer cells and so on.In this study,we induced the TLR2/NFκB pathway induced by P.freudennreichii in mouse colon cancer cells,and the molecular mechanism of the action of P.freudennreichii on mouse colon cancer cells was investigated.MTT assay combined with flow cytometry,The results showed that P.freudennreichii had an inhibitory effect on mouse colon cancer cells and promoted the apoptosis of mouse colon cancer cells,But the apoptotic rate was much lower than that of Escherichia coll Using EIISA method to detect the effect of P.freudennreichii on mouse colon cancer cells,The levels of IL-2,IL-6,IL-8 and IL-12 were increased with the increase of the concentration of P.freudennreichii,IL-10 and TNF-a were significantly increased with the increase of the concentration of P.freudennreichii,But the content of IL-4 was significantly decreased.Real-time PCR combined with Western Blot detection found that.The mRNA expression level and protein expression of TLR2,NFκB and TNF-α increased with the increase of the concentration of P.freudennreichii.But MyD88 mRNA expression levels and protein expression did not change.The results show that:activation of TLR2/NFκB pathway by P.freudennreichii,Activation of NFκB by TLR2 promotes the production of various inflammatory factors in mouse colon cancer cells.The results of this study confirmed that P.freudennreichii can promote apoptosis of mouse colon cancer cells,could activate the TLR2/NFκB pathway to promote mouse colon cancer cells produce a variety of inflammatory factors,However,Need to be confirmed P.freudennreichii promotes the apoptosis of mouse colon cancer cells,whether it is related to the activation of TLR2/NFkB pathway that produce a large number of inflammatory factors...