| Objective:Systematic study on the chemical constituents of petrole--um ether Fraction（Chirita linearifolia W.T.Wang）.This work can la--y a foundation for the future activity compounds screening（to reveal the material foundation of its bio-activities）,and further to provide an e--vidence for exploitation and utilization of this medicinal plant.Methods:The crude powder of the whole plant was extracted with ethanol by Low-temperature refluxing to give a total ethanol extract.T--he ethanol extract was suspended in water and extracted by petroleum--ether,ethyl acetate and n-butanol subsequently to obtain petroleum eth--er fraction,ethyl acetate fraction,n-butanol and the remaining parts ofwater.The methods of silicagel column chromatography,Sephadex LH-20column chromatography and recrystallization,were used to isolate and p--urify chemical constituents from petroleum ether.The spectroscopic me-thods(EI-MS,1H-NMR,13C-NMR,DEPT)and physical and chemical tests w ere use-d to identify the structures of the obtained compounds.Results:Twenty compounds were isolated from the ethyl petroleu--mether Fraction（Chirita linearifolia W.T.Wang）.And sixteen of the--m have been identified as 2-methyl-anthraquinone（1）,2-ethyl-anthraquin--one（2）,heptacosanoic acid（3）,β-sitostenone（4）,1-hydroxy-2-methyl-9,10-ant--hraquinone（5）,1-hydroxy-octyl-9,10-anthraquinone（6）,9-octadecenoicacid-2’,3’-dihydroxypropyl ester（7）,（R）-7-hydroxy-α-dunnione（8）,1-hydroxy-2-hydr--oxymethy lanthraquinone（9）,vanillic acid（10）,glycerin-docos-anate（11）,dau--costerol（12）,β-D-Glucopyranoside,2-（3,4-dihydroxyphenyl）ethyl,4-[（2E）-3-（4-hy--droxy-3-methoxyphenyl）-2-propenoate]（13）,ursolic acid（14）,sitoster--ol（15）,heitziethanoid A（16）.Conclusion:The above compounds were mainly anthraquinones,gl--ycerides and phenylethanosides:All of compounds were isolated from the plant for the first time.Compound 1,2,3,5,6 and 9 were anthraquin--ones.Compound 7 and 11 were glycerides.Compound 13 was phenyl--hanosides. |
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