Establishment Of Lateral Flow Assay Based On Goldmag Nanoparticles For The Detection Of Prostate Cancer Related Markers And Investigation Of The Relationship Between Markers

Prostate cancer(PCa)is a common malignant tumor in the male urinary system.Most of patents was found as terminal prostate cancer in their first-diagnosis because of the asymptomatic property of this cancer.In China,the prostate cancer has exhibited an increased morbidity in recent years.Prostate-specific antigen(PSA)screening and the association study of prostate cancer and kallikrein 3(KLK3)gene(rs2735839 and rs 17632542)polymorphism have drawn great attention.In this study,by using GoldMag nanoparticles as carrier,we established magnetic quantitative lateral flow assay for PSA testing and PCR-lateral flow assay for KLK3 rs2735839,rs 17632542 genotyping.To clarify the effectiveness and applicability of these detection systems,the specificity,sensitivity and stability of the systems were evaluated and some clinical samples were assessed.Main results:1.For the magnetic quantitative lateral flow assay system,the conjugation of PSA antibody to GoldMag nanoparticles,the concentration and amount of PSA antibody in test line and conjugate pad have been optimized.Compared with traditional optical assay methods,this detection system shown good repeatability,accuracy and specificity in PSA quantification.2.Through the construction of positive controls,the selection of specific primers and optimization of reaction system,PCR-lateral flow assay platform was established for rapid determination of SNPs of rs2735839 and rs 17632542 in KLK3 gene,respectively.A good concordance was found between this detection system and DNA sequencing.3.The evaluation of magnetic quantitative lateral flow assay and PCR-lateral flow assay with clinical samples indicated that rs2735839-G in KLK3 gene is related with higher PSA level.Due to the simple operation,short analysis time and widely application,magnetic-and PCR-lateral flow assays based on GoldMag nanoparticles,performed as reliable,accurate detection methods for PSA screening and KLK3 genotyping,respectively.