| Objective:The model of isoprenaline-induced chronic heart failure(CHF)in rats was established in this present research.The rats are divided into the treatment group and the control group,which are respectively treated with anti-heart-failure Ⅰ and benazepril.The mechanism of the anti-heart-failure Ⅰ in treating CHF was explored through comparing the different effects between the two groups,including rats cardiac functions,CHF index,the influence of oxidation factor and serum inflammatory factor,and the protein expression of myocardial tissue.Methods:Using isoprenaline(ISO)subcutaneous injection method,the CHF rat model was established.The rats,which were injected isoprenaline,were randomly divided into four groups including the model group,the benazepril group,the anti-heart-failure Ⅰ group,the anti-heart-failure Ⅰ and benazepril group,and the negative group.After 4 weeks,the myocardial ultramicrostructures,serum b-type natriuretic peptide,nitric oxide,angiotensin Ⅱ,tumor necrosis factor a,interleuki-6,the superoxide dismutase(SOD)and Malondialdehyde(MDA)were respectively observed with the HE staining and Elisa.The expressions of proteins XBP1-s,the influence of GRP78,CHOP,which are related with the apoptotic pathway of myocardial tissue of endoplasmic reticulum stress treated with anti-heart-failure Ⅰ.The mechanisms of anti-heart-failure Ⅰ in CHF were investigated from the above aspects.Results:The tested four groups can effectively reduce the disorder degree of myocardial structure and inflammatory cells infiltration.In particular,the curative effect of the anti-heart-failure Ⅰ and benazepril group was best.Compared with the model group,the anti-heart-failure Ⅰgroup showed an decrease in contents of serum of BNP,Ang Ⅱ,TNF-α and MDA,and an obvious similarity in expressions of proteins XBP1-s,GRP78 and CHOP(P<0.05).Compared with the benazepril group,the anti-heart-failure Ⅰ group had no statistical difference in reducing the serum BNP,Ang Ⅱ,TNF-α,IL-6,MDA,and the related protein expression of CHOP,GRP78 and XBP1(P>0.05).Besides,the statistical difference between the benazepril group and the anti-heart-failure Ⅰ group can been observed in the reducing CO and the increasing SOD(P<0.05).Conclusion:Anti-heart-failure Ⅰ can reduce the cardiac markers of CHF rats,its effect may be related to anti-inflammatory,anti-oxidant,anti-apoptosis. |
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