Chemical And Bioactive Studies On Folium Mori And Ramulus Mori

Morus alba L.,which belongs to the genus Morus of Moracea,is one of the traditional herbal medicines.Its root bark,branch,leaf,and fruit can be used as medicines.This genus contains a variety of phenolic compounds including Diels-Alder adduct compounds,isoprenylated flavonoids,2-arylbenzopyrans and alkaloids.Modern medical studies of Morus alba have reported antioxidant,anti-diabetic,anti-hypertensive,antiviral,and anti-inflammatory effects.The systematic phytochemical study on Folium Mori and Ramulus Mori yielded fifty-eight compounds.Thirty-five compounds were isolated from Folium Mori,and the structures were elucidated as wittifuran X(1),3’,5’-dimethoxy wittifuran Y(2),wittifuran Y(3),wittifuran Z(4),5-hydroxyethyl moracin M(5),moracin E(16),moracin I(17),moracin M(18),moracin N(19),moracin X(20),moracin C(21),moracin D(22),moracin V(23),morusyunnansin I(6a,6b),morusyunnansin H(7a,7b),morusyunnansin J(8),morusyunnansin K(9),morusyunnansin L(10),morusyunnansin M(11),morusyunnansin N(12),(2S)-7,2’-dihydroxy-4’-methoxy-8-prenylflavan(13),(2S)-2’,4’-dihydroxy-7-methoxy-8-prenylflavan(14),2’,4’-dihydroxy-7-methoxy-8-hydroxyethylflavan(15),morachalcone A(24),isobavaehaleone(25),2’,4’.4,2"-tetrahydroxy-3’-[3"-methylbut-3"-enyl]-chalcone(26),2,4,2’,4’-tetrahydroxychalcone(27),euchrenone a7(28),morin(29),kaempferol(30),norartoearpetin(31),diisobutyl phthalate(32),dibutyl phthalate(33),loliolide(34),9,16-dioxo-10,12,14-octadecatrienoic acid(35).Compounds 1-12 were new compounds.The cytotoxic activity was screened by using MTT assay,and the results showed that compounds 13,18,21,30 were toxic to A549 and NCI-H292 cells on different levels.Twenty-three compounds were obtained from Ramulus Mori,including moracin P(36),wittifuran V(37),moracin M-3’-O-β-D-glucopyranoside(38),mornigrol E(39),mornigrol F(40),kuwanon C(41),2,3-trans-dihydromorin(42),2,3-cis-Dihydromorin(43),7-hydroxycoumarin(44),scopoletin(45),isoscopoletin(46),2,5-dimethoxy-1,4-benzenedicarboxaldehyde(47),2,4-dihydroxybenzaldehyde(48),p-hydroxybenzaldehyde(49),vanillin(50),syringaldehyde(51),syringic acid(52),2,5-dihydroxy-benzoate(53),trans-p-coumaric acid(54),3-(4-ethoxyphenyl)-2-propenoic acid(55),adipic acid(56),p-sitosterol(57),2,4-hexadienedioic acid(58).