Significance Of Detecting IGRP-specific T Cell In Blood Of Type 1 Diabetes Mellitus

Objective:To explore whether IGRP-specific T cell can be detected in peripheral blood of Type 1 Diabetes Mellitus,and whether IGRP is a kind of autoantigen playing a pathogenic role in the onset of the disease.Methods:10 T1DM patients and 10 healthy controls matched with age and sex were enrolled in our study and their periphera blood mononuclear cells(PBMC)were isolated.We implemented ELISPOT(Enzyme-linked Immunospot Assay)to detect the interferon-y(IFN-γ)which secreted by IGRP-specific T cell.Results were compared between these two groups.Results:There were 5/10 patients have positive reactivation against the IGRP epitopes in our study.The positive rates is 50%.While all healthy controls have no reactivation against the epitopes,which was significantly different from T1DM(P<0.05).Conclusions:IGRP can cause T cell proliferation in Peripheral Blood of Type 1 diabetes patients,while healthy controls have no response.So IGRP may be one of autoantigen inducing autoimmune attack in T1DM.