Study On Curcumin-induced Apoptosis And Its Mechanism In Human Endometrial Cancer Cells (HEC-1-B)

BackgroundEndometrial carcinoma (also known as uterine cancer) is one of the common malignant tumors in female reproductive system. According to statistics, the prevalence of endometrial cancer in Europe and the United States is the highest.The prevalence rates in Asian countries are also increasing. Now the principle of treatment based on surgery and radiotherapy, for fertility patients or patients with late recurrence need adjuvant hormonal therapy, chemical and immune therapy, however, chemotherapy and hormone therapy drugs have obvious contraindications and side effects. Therefore, To find a safe, reliable, efficient and widely used new drug for anti endometrial cancer is a hot spot of research.ObjectiveObjective To observe the effect of curcumin on human endometrial cancer cell (HEC-1-B) and the expression of related proteins and to investigate the apoptosis mechnisms proliferation of human endometrial carcinoma(HEC-1-B) cells in vitro.MethodsHEC-1-B cells cultured in vitro were randomly divided into 5 groups:control group, curcumin group, Ⅰ,Ⅱ,Ⅲand IV. Curcumin were given different concentrations (0,10,20, 40,80) μmol/Lwith culture time of 48h and 72h.1) The MTT method was used to detect the growth inhibition rate of HEC-1-B.2) The cellnu clide form of HEC-1-B was detected by Hoechest33258 fluorescence staining.3) With western blot method the expression level of related protein was detected.Using SPSS11.5 statistics software to do statistical analysis of the above test results was made.Results1. The effect of curcumin on the proliferation and apoptosis of HEC-1-B cells(1) MTT experiment results show that, absorbance (OD)in all curcumin groups is lower than the control group (P< 0.01), and in a certain concentration (10-80) μmol/L range it is dose dependent, suggesting that curcumin could inhibit the proliferation of HEC-1-B cells.(2) the results of apoptosis showed that the apoptosis rate of HEC-1-B cells was significantly increased (P<0.01), and the concentration (10～80) was dose dependent in a certain concentration (μmol/L). Fluorescence staining was observed in the control group, and the cell morphology was observed in the control group, which showed the characteristics of apoptosis, such as nuclear fragmentation, nuclear condensation, fluorescence intensity enhancement. Curcumin could induce apoptosis of HEC-1-B cells.2.The effect of curcumin on related protein expression of HEC-1-BWestern blot method shows that in comparison with the control group, different concentrations of curcumin after 48h and 72h, HEC-1-B cells survivin protein expression level were significantly lower than those in control groups of different concentration decreased, and with curcumin concentration and time increasing and decreasing, in a time and dose-dependent manner (P< 0.01). Western blot was used to detect the expression of caspase-3 protein, compared with the control group, different concentrations of curcumin after 48h and 72h, HEC-1-B cells caspase-3 protein expression level were lower than those in the control groups with varying degrees of increase, and decreases with the increase of curcumin and time and increased in a time and dose dependent manner (P< 0.01).ConclusionCurcumin has the effect of inhibition on human HEC-1-B in vitro, and the inhibition is in a time-dose-dependent manner in a certain concentration range. In a certain range of concentration it can inhibit the growth of HEC-1-B cells and induce its apoptosis. the mechanism may be expression of endometrial carcinoma HEC-1-B cells survivin protein, direct activation of Caspase-3 and induced apoptosis of HEC-1-B cells.