The Research On The Mechanism Of Hypoglycemic Effect Of Fucoxanthin

The hypoglycemic effect and mechanism of fucoxanthin was studied in diabetic rats during this investigation.A model of type 2 diabetes mellitus(T2DM)was established by feeding high-fat diet and injecting streptozocin(STZ)via male SD rats.Then the diabetic rats were divided into three dosage groups[5,10,20 mg/(kg·bodyweight·d)],which were given a daily dose for 4 weeks respectively.The level of fasting blood-glucose(FBG)was meansured every week and oral glucose tolerance test(OGTT)was tested in the 4th week.Some of the biochemical indexes including glucose serum protein(GSP),inslin(INS),Alanine Aminotransferase(ALT),aspartate aminotransferase(AST),blood urea nitrogen(BUN),creatinine(Cr)were tested to verify the effect of fucoxanthin on diabetic rats.The cellular morphology of liver tissue was also observed under microscope.After that,the expressions of the genes that involved in PI3K/AKT pathway were tested though RT-PCR and Western bolt.The results suggested that after 4-week-treatment with fucoxanthin,compared to the model group,the weight of the dosage groups increased significantly and the viscera index tended to be normal.The level of FBG,GSP,FINS were remarkably lower in fucoxanthin treated groups.The level of AST,ALT,BUN and Cr in serum exhibited a decreasing tendency in fucoxanthin treated groups.And the damaged liver cells in diabetic rats tend to heal after treatment.The results of RT-PCR and Western bolt of the liver and muscle tissue showed that after 4-week-treatment with fucoxanthin,compared to the model group,the expression level of GSK-3β was remarkably lower,and the expression level of GS was remarkably higher in liver tissue;The expression level of GSK-3β was remarkably lower,and the expression level of GS,GLUT4 was remarkably higher in muscle tissue.All these consequences revealed that fucoxanthin had a positive effect on regulating PI3K/AKT pathway,which can accelerate the synthesis of glycogen,and promote the interacellular transport of glucose.So fucoxanthin has its hypoglycemic effect by improving the body’s insulin sensitivity.