Study Of Liposomes Preparation And Quality Of Cyclovirobuxine D

ObjectiveHuanyangning tablet,which major contain cyclovirobuxine D(CVB-D),was used to treat cardiovascular diseases in clinical.However,multiple dosage regimen was requested for its low water solubility and bioavailability.This study aimed to establish the preparation process of cyclovirobuxine D liposomes to increase the bioavailability..MethodHPLC and acid dye colorimetry methods were established to analyze the content of CVB-D.The preparation process of CVB-D liposomes was optimized through the selective of solvent,content of phospholipid,ratio of phospholipids and cholesterol,ratio of drug and phospholipids,cement hydration temperature,cement hydration time,ultrasonic way and ultrasonic time.What’ s more,the qualities of CVB-D liposomes were control based on the measurement of morphology,partical size,zeta-potential,permeability and release rate in vitro.ResultFor the complex pretreatment,HPLC method wasn’ t suitable to measurement of the content of CVB-D.Whereas acid dye colorimetry method was observed to have great specificity,precision,stability and recovery rate in measurement of the content of CVB-D,and its conform to the rules of methodology.The optimal preparation of CVB-D liposomes,which could rise the encapsulation efficiency to 85%,were shown as follows.Phosphatidylcholine:cholesterol = 7:1;Content of phosphatidylcholine is 15 mg/ml.Phosphatidylcholine:CVB-D=20:1.Film-forming temperature,is 40℃.Hydration time is 60 min.Sonication time is 3 min.The particle size and zata-potential of the CVB-D liposomes were 174.3±6.113 nm and-22.2 mv,respectively.According to the permeability experiment,the half-life period of the CVB-D liposomes was 207.1 days,and the retention rate was 77.9%and 84.9%,when the liposomes was stored at 25℃,1 atm and 4℃,1 atm,respectivly.In vitro releasement experiment showed that 72.3%of drug were released from CVB-D liposomes in 2 h,which was much higher than 47.9%in CVB-D crude drug.ConclusionThe methods of CVB-D content measurement and its liposomes preparation were successfully established.And the product was presented with stability quality and high drug release rate.