Effects Of Rosuvastatin On Apolipoprotein J Expression In Rats Following By Carotid Artery Balloon Injury And The Relationship Between Apolipoprotein J And Restenosis

Objective: Establish a restenosis model of rat carotid artery, and observe Apolipoprotein J expression in rat injury carotid artery, discusses the relationship between Apo J and vascular restenosis with rosuvastatin intervened.Methods: 96 wistar rats were divided into control group, model group and rosuvastatin group,the establishment of animal model in the control group and model group and detecting the expression of Apo J have been done. On the basis of this study do the further research with rosuvastatin intervened. The road map as figure 7. The 2F Fogarty balloon catheter was inserted into the carotid artery in model group and rosuvastatin group, and daily felling 10 mg/kg rosuvastatin to fill the stomach 1 day before the establishment in rosuvastatin group.The control group received sham-operation.Three groups respectively at 1, 2, 3, 4 weeks take venous blood, put to death in the rat and take out the right common carotid artery segment, immediately frozen in liquid nitrogen, and turn to- 80 ℃refrigerator, standby for biochemical tests.Using enzyme-linked immunosorbent assay(ELISA) method to detect the concentration of serum Apo J, real- time PCR method to detect carotid artery in the Apo J m RNA expression level, Western blot method to detect Apo J protein expression in the common carotid arteries, HE staining to observe the morphological changes of the common carotid arteries.Results: Through the carotid artery balloon injury to establish animal model of restenosis, this method is simple and practical, strong repeatability, this research building a total of 32, live 30, the survival rate was 93.75%, and survival was observed in rats of arterial intimal injury morphology, restenosis model building success.(1)ELISA:rosuvastatin group Apo J density is no statistical difference before and after operation.(2)the real-time PCR: three groups all can be detected in the Apo J m RNA expression, rosuvastatin group at every time point of Apo J m RNA expression of measuring tool was statistically difference, and to peak in 2rd week;Compared with model group, Apo J m RNA expression of statistically significant in 1st, 2nd week.(3)Western blot: three groups all can be detected in the Apo J protein expression, rosuvastatin group of each time point Apo J protein expression was statistically difference, and with the expression significantly in 2nd, 3rdweek, and to peak in 2rd week;Compared with model group, Apo J m RNA expression of statistically significant in 1st, 2nd week(P < 0.05).(4)HE staining: lining of membrane area ratio(I/M) indicates the degree of endometrial thickening: The I/M close to 0 in control group, and compared with the model group and rosuvastatin group at each time point, were statistically significant; I/M to the maximum in 4th week;The rosuvastatin group four time points of I/M, with statistical difference, The rosuvastatin group compared with model group in 4th week has significant difference statistically significant.Conclusion: This building method with the rat carotid artery balloon injury has high survival rate. Research to the Apo J in the expression level of the different time points, found rosuvastatin can inhibit intimal hyperplasia and promote the expresstion of Apo J in the injury carotid artery, suggesting the action of rosuvastatin inhibiting the vascular restenosis may be associated with the expression quantity of raised Apo J. Apo J may have inhibitory effect on vascular restenosis, but the mechanism needs further research.