Preparation Of New Organic Nano-Antibacterial Agents And Their Application In Advanced Antibiotic Medical Dressing

In this study, H-type polymerizable cationic surfmer N-(2-(methacryloyloxy)ethyl)-N,N-dimethylhexadecan-1- aminium bromide(H(16)) and N-(2-(methacryloyloxy)ethyl)-N,N-dimethyldodecan-1- aminium bromide(H(12)) have beenprepared by dimethyl amino ethyl methacrylate(DMAEMA), bromo-hexadecane(HB) and bromo dodecane(DB), respectively; T-type polymerizable cationic surfmer N-(2-(methacryloyloxy) undecyl)-N,N-dimethylethyl-1- aminium bromide(T(11)) has beenprepared by methacryloyl chloride, 11-bromo-1-ol and dimethylethylamine. These three polymerizable cationic surfmerpolymerized with styrene(St) and methyl methacrylate(MMA), to obtain a series ofcationic nanopolystyrene(CNPS) and cationic nanopolymethylmethacrylate(CNPM), respectively.The structure of resultant nanopolymers was confirmed by 1H NMR and FT-IR spectroscopy.The molecular weight and thermal stability were studied by gel-permeation chromatograph(GPC) and thermogravimetric analysis(TGA).The polymerization kinetics indicated that such copolymerization was fast microemulsion polymerization reacitonsand monomers can be polymerizationcompletely.The measurementof zetapotential and particle size in different polymerization time show that they do not change with the increase of reaction degree.In comparison of CNPS, the copolymer with a longer alkyl chain H(16) has better antimicrobial than H(12); The copolymer with H-type H(12) or T-type T(11) has the similar antibacterial effect; CNPS have better antimicrobial than CNPM with the same polymerizable cationic surfmer H(16); When the two type surfmer H(16) and T(11) mixture used as an emulsifier copolymerization with St, it does not appear the significant synergies, in other words the copolymers antibacterial activity has not been significantly improved; The nanopolymers’ antibacterial activities increased as the polymerizable cationic surfmer increased in recipe and antibacterial effect against S. aureus is better than E. coli.In vitro cytotoxicity test for CNPS-4 and CNPM-4 indicate the two polymers have no cytotoxicity. When add them into the alginate gel film, or load on the alginate fiber dressing, which show a certain antibacterial effect against to S. aureus and E. coli, and can kill all bacteria when the addition concentration reaches a certain value. The authoritative testing organizations test the antibacterial rate of the fiber antibacterial dressing reached 99.9% against S. aureus, E. coli and P. aeruginosa, and 99.7% against Candida albicans, shows excellent and broad spectrum antimicrobial effect.Using New Zealand white rabbit scald wounds as experimental model, compared with none-added antibacterial agent alginate fiber dressing and traditional gauze dressing, to evaluate the effect on wound healing about the homemade anti-infection fiber dressing. The results show that the homemade anti-infection fiber dressing can prevent adhesion and avoid secondary damage to the wound. The new dressing showed a certain advantages to promote wound healing, include reduce the frequency of dressing change, healing cost and nursing workload.