| Objectives The aims of this study were to compare the levels of serum LPS and other related indicators in patients with nonalcoholic fatty liver disease(NAFLD) and that in non NAFLD control individuals。We investigated the relationship between serum LPS and the influence factors of NAFLD to provide certain reference value for the clinical prevention and treatment of NAFLD.Methods The research was designed as a case-control study. 59 patients with NAFLD and 59 control subjects were enrolled in this study.In the NAFLD group, there are 38 males and 21 females. 36 males and 23 females were included in the control group. The weight and height of the patients were measured with a calibrated scale after removing shoes and heavy clothing, if any. The information of blood pressure, body mass index(BMI) and mean artery pressure(MAP) of the subjects were all collected. Venous blood samples were obtained from the subjects after an overnight fast(12 h) for the measurement of TP(total protein), ALB(albumin), ALT(alanine transaminase), AST(serum aspartate transaminase), TC(total cholesterol), LDL cholesterol, HDL cholesterol, TG(triglyceride), UA(uric acid) and FBG(fasting blood glucose). All laboratory biochemical parameters were measured in a conventional automated analyzer. we measure the serum LPS level using the method of coloration matrix. Simple comparison of the clinical data between case and control groups was carried out using the χ2-test, Chi square test or t-test.The relationship between serum LPS level and NAFLD was analyzed by stratified method, and the risk factors and protective factors of NAFLD were analyzed by Logistic regression analysis. The probability of difference between variables was considered statistically significant if 2-sided P value was less than 0.05.Results 1 There was no significant differences in compositions of sex,average age and average height between the NAFLD group and the control group(p>0.05), and has good comparability of themselves. The weight, BMI, blood pressure, MAP, ALT, LDL-c, and FBG in NAFLD group were significantly higher than those of control group(p<0.05). The level of HDL-C in NAFLD group were significantly lower than those of control group(p<0.05). 2 The level of LPS in patients with NAFLD was significantly higher than that of control group(p<0.05), and in NAFLD group, the ratio of higher serum LPS level(LPS≥0.41EU/m L) was significantly higher than that of control group(x2=5.735,p<0.05,OR=2.453,95%CI:1.170-5.143). 3 In females, the ratio of higher serum LPS level was significantly higher than that of control group(x2=7.662, p<0.05, OR=2.844, 95%CI: 1.345-6.012), but that in men was no difference(p>0.05). When age≥45 years old or age<45 years old, the ratio of higher serum LPS level has no difference between the two groups(p>0.05). When BMI≥25kg/m2, the ratio of higher LPS level was significantly higher than that of control group(x2=7.878, p<0.05, OR=5.900, 95%CI: 1.546-22.517), but there was no difference when BMI<25kg/m2(p>0.05). 4 There was no significant difference in serum LPS levels between the NAFL group and the control group(p>0.05). Compared with control group, NAFL group had a higher level of serum LPS, and the ratio of higher serum LPS level was significantly higher than that of control group(x2=11.813,p<0.05,OR=5.263,95%CI:1.965-14.096). Compared the level of serum LPS between the NAFL group and NASH group, there was no significant difference(p>0.05). 5 Multivariate Logistic regression analysis showed that BMI, TG, HDL-c were related to NAFLD, and serum LPS level had no significant correlation with NAFLD.Conclusions 1 The NAFLD patients had a higher level of serum LPS, and the ratio of high serum LPS level was increasing. 2 In females, the ratio of high serum LPS level was significantly higher in NAFLD patients, but that in men was no difference. When age≥45 years old or age<45 years old, the ratio of high serum LPS level has no difference in NAFLD patients. When BMI≥25kg/m2, the ratio of high LPS level was increasing in NAFLD patients, but that was no difference when BMI<25kg/m2. 3 Multivariate Logistic regression analysis showed that BMI, TG, HDL-c were related to NAFLD, and serum LPS level had no significant correlation with NAFLD. |
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