The Expression, Clinical Significance And Function Of MicroRNA-214 In Pancreatic Cancer

Objective: To observe the expression of miR-214(micro RNA-214) in pancreatic cancer and explore its clinical significance and influence on pancreatic cancer cell lines’ biological behaviors.Methods: 1.Real-time PCR was used to detect the miR-214 expressions between pancreatic cancer tissues and matched adjacent tissues, human pancreatic cancer cell lines and normal pancreatic ductal epithelial cells, respectively.The correlations of miR-214 expression with clinic-pathological factors and clinical prognosis were analyzed.2.MiR-214 inhibitors and miR-214 mimics were transfected into PANC-1 cells by LipofectamineTM 2000. Real-time PCR was used to detect the miR-214 expressions.The cells Proliferation was measured by CCK-8 assay. Wound healing assays were performed to examine the migration ability of PANC-1 cells.Results: MiR-214 expression was up-regulated in 69.4 %(25/36) of tumor tissue specimens. The relative expression level of miR-214 was significantly higher in tumor tissues than in matched adjacent tissues(3.45 vs 1.52, Z=-3.82,P<0.01). Higher miR-214 level was strongly associated with T3/T4 stage(P = 0.018). The Kaplan-Meier analysis reveals that patients with higher expression of miR-214 had a shorter survival time(P=0.032). Compared with negative control, transfection with miR-214 inhibitors could significantly decrease the expression of miR-214,the proliferation and migration ability of PANC-1 cells(both P < 0.01). On the other hand, transfection with miR-214 mimics could improve the expression of miR-214,the proliferation and migration ability of PANC-1 cells(both P < 0.01)Conclusion: The expression of miR-214 is associated with clinic-pathological features, patient’s clinical prognosis and biology behavior of pancreatic cancer cells. So it may be used as a potential therapeutic target, a diagnostic biomarker and a prognostic predictor in patients with pancreatic cancer.