Relationship Between IFN-γ And TNF-α Gene Polymorphism And Oral Lichen Planus And Their Effects On The Expression Of IFN-γ And TNF-α

Objective: To study the relationship between IFN-γ and TNF-α gene polymorphism and oral lichen planus and their effects on the expression of IFN-γ and TNF-α.Methods: 1.Collected 42 cases of patients with oral lichen planus(experimental group) and 72 cases of natural and healthy people(control group) in peripheral blood, DNA extraction, by PCR sequencing, detection of IFN-γ(rs2430561) and TNF-α(rs1800629) two gene polymorphism genotype, analysis and comparison of IFN-γ(rs2430561) and TNF-α(rs1800629)genotype distribution.2.By ELISA to detect the expression of serum IFN-γ and TNF-α situation,analysis and comparison of type AA,AT and TT patients with IFN-γ(rs2430561) and type AA.AG and GG type patients with TNF-α expression levels.Results: 1.Compared with the normal control group, there was no significant difference in the frequency distribution of genotype frequencies and allele frequencies of the two single nucleotide polymorphisms in the experimental group and the control group OLP(p>0.05). TNF-α(rs1800629)A allele is detected in erosive, reticulate type did not detect the A allele.2.In experimental group, the serum levels of IFN-γ and TNF-α were significantly lower than those in normal control group(p < 0.05); among them, erosive serum level of IFN-γ and TNF-α slightly higher than the reticulate type, the difference was not significant(p> 0.05).3.The serum level of IFN-γ in type TT of OLP patients with IFN-γ(rs2430561) is higher than the patients of type AT(127.02ng/L>115.36ng/L),but there was no significant difference(p>0.05).4.The serum level of TNF-α in GG type of OLP patients with TNF-α(rs1800629) was significantly higher than that of GA type(256.25 ng/L>128.75ng/L). GA type was not detected in patients with reticulate type.Conclusion:1.TNF-α(rs1800629) gene polymorphism may not be the independent factors influencing the OLP, A allele may promote the expression of serum TNF-α, associated with the OLP illness severity.2.IFN-γ(rs2430561) locus T allele may promote the expression of serum levels of IFN-γ.3.IFN-γ(rs2430561), TNF-α(rs1800629) gene polymorphism may be associated with the pathogenesis of OLP, disease progression and the severity of inflammation, but the relevant mechanisms need to be further studied.