Study On The Expression Of ADA And TB-Ab In Primary Infertility With Latent Tuberculosis Women’s Menstrual Blood

Objective: This article used enzyme-linked immunosorbent assay(ELISA) to detect adenosine deaminase(ADA) and tuberculosis antibody(TB-Ab) concentration in menstrual blood, real-time fluorescence quantification polymerase chain reaction(PCR) to measure mycobacterium tuberculosis DNA concentration in endometrium. What’s more, we collected associated clinical data, then discussed the expression levels of ADA、TB-Ab in primary infertility with latent tuberculosis women’s menstrual blood, and whether they had the predictive value for genital latent tuberculosis in infertility, including ADA,TB-Ab in menstrual blood, chest X-ray results and TB-IGRA、CA125、ESR in peripheral blood.Methods: We collected 165 infertile women’s menstrual blood and endometrium who were treated with assisted reproductive technology at Inner Mongolia university reproductive center from March 2014 to October 2015. All patients were divided into two groups by the results of TB-IGRA. The experimental group was patients with TB-IGRA positive and the main cause of infertility was primary tubal factor. The control group was patients with TB-IGRA negative and the main cause of infertility was primary male factors. All the research objects were all less than 40 years old,no clinical symptoms of TB, excluded active tuberculosis by imaging examination and endometrial tissue pathology examination. We used enzyme-linked immunosorbent assay(ELISA) to detect the concentration of adenosine deaminase(ADA) and tuberculosis antibody in menstrual blood, real-time fluorescent quantitative polymerase chain reaction(PCR) to detect mycobacterium tuberculosis DNA content in the endometrium, and collected the patients’ clinical data, such as TB-IGRA, TB-Ab, CA125, chest X-ray result. Then comparative analysis the difference of experimental and control group in ADA, TB-Ab, CA125, ESR and chest X-ray results. What’s more, we analyzed the positive rate of tuberculosis in endometrium.Results:(1) The experimental group’s ADA, TB–Ab,CA125 values are significantly higher than that of control group(P<0.05).(2) Blood sedimentation, the X-ray results of experimental group and control group have no significant difference(P>0.05).(3) All the groups of ADA and TB-Ab numerical values are positive correlation(r=0.274, P=0.001).(4) The experimental line endometrial tuberculosis real-time fluorescent quantitative PCR positive rate is very low.Conclusion:(1) The high expression of ADA level in the menstrual blood maybe a prediction index of female genital latent tuberculosis.(2) The high expression of TB-Ab level in the menstrual blood maybe a prediction index of female genital latent tuberculosis.(3) The menstrual blood of ADA and TB- Ab level has a certain positive correlation, both high expression level maybe used as a prediction index of female genital latent TB.(4) CA125 values in the peripheral blood increasing indicate existence of inflammation; maybe have an assisted diagnosis value of female genital tuberculosis inflammation.(5) Through this experiment, we considered that the positive rate of endometrial tuberculosis in latent tuberculosis infections, detected by real-time fluorescent quantitative PCR was very low. It is necessary to explore a new detective method to exam mycobacterium tuberculosis DNA in genital latent tuberculosis women’s endometrium.