| Objective:In the first part of this study, through the establishment of rats, we took periodontal interventions in rats, and tested the expression of C- reactive protein, to explore the influence of different periodontal interventions on the atherosclerosis. In the second part of this study,we builded the rat model of diabetes mellitus(DM) and CP and the compound. Methods:In the first part, 100 rats were randomly divided into: group A(normal control group, 7), group B(the As group, 8), group C(the CP group, 35), group D(As + CP group, 50). Group C and group D were randomly divided respectively, according to the different periodontal interventions, including C1 / D1 group(the natural process group), C2 /D2 group(the mechanical periodontal treatment group), the C3 / D3 group(the drug therapy group), C4 / D4 group(the tooth extraction group); The C4 / D4 group were divided into C4-1 / D4-1 group(the pure tooth extraction group) and C4-2 / D4-2 group(tooth extraction + antibiotics group), and D2 group were divided into D2-1 group(the pure mechanical treatment group), D2-2 group(mechanical treatment + periocline group), D2-3 group(mechanical treatment + periocline + antibiotics group). 9 week after successful modeling executed rats, detect the CRP levels of the carotid artery organization by immunohistochemical method.In the second part, 19 male SD rats were randomly divided into: group A(the normal control group, 1), group B(DM group, 8), group C(DM + CP group, 10). Group B, group C high fat and sugar feed 2 months, then intraperitoneal inject streptozotocin(STZ) to build DM model, and at the same time, group C were taken CP modeling processing. Results:In the first part, CRP immunohistochemical results: the depth of the CRP positive staining of group B, group C1, group D1(the natural process group) were significantly higher than that of group A. The depth of the CRP positive staining in group C2, group C3 and group C4-2 were lower than group C1, and among them, group C2 was weakest, but group C4-1 was higher than group C1. The depth of the CRP positive staining in group D2-1, group D2-2, group D2-3, group D3, group D4-1 and group D4-2 were lower than group D1, group D2-3 was weakest. The depth of the CRP positive staining in group D2-3, group D2-2, group D2-1 deepening in turn. The depth of the CRP positive staining in group C4-2/ D4-2(tooth extraction + antibiotics group) was lower than corresponding group C4-1/D4-1(pure tooth extraction group). The rank of the CRP positive staining is:C4-1>B>C1>C3>C4-2>C2>A,D1>D4-1>D3>D4-2>B>D2-1>D2-2>D2-3>A.In the second part, DM and CP modeling results: the final fasting glucose of rats in group B and group C were greater than 11.2mmol/L, tendency for L, and the rats showed obvious symptoms of diabetes. The rats in group C showed typical chronic periodontitis symptoms. Conclusion:1. No matter with or without atherosclerosis in chronic periodontitis SD rats, nature development will make the CRP expression in carotid artery increased,then will increase the risk of As. Periodontal intervention have certain improvement effect on the expression of CRP in rat carotid artery, which may reduce the risk of As. Compare with pure mechanical periodontal treatment, periodontal mechanical treatment with local anti-inflammatory drugs and anti-inflammatory drugs have more significantly improvement effect on the local expression of CRP in the rat carotid artery, and can get a better curative effect. Do not control periodontal inflammation, pure tooth extraction treatment can increase the local expression of CRP in the rat carotid artery, and increase the risk of the occurrence and development of atherosclerosis, tooth extraction at the same time giving the anti-inflammatory treatment can significantly reduce the risk.2. Diabetes model and diabetes with chronic periodontitis composite model were successful. |
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