The Role Of Biofilm In Clinical Diagnosis Of Adenoid Hypertrophy In Children

Adenoid hypertrophy is one of the most frequently occurring diseases of the nose and throat in children with ear, neck and head and neck surgery. It is usually caused by pathological changes. It is often caused by various factors. A large number of studies at home and abroad have proved that adenoid hypertrophy and its complications are closely related to the chronic infection caused by biofilm formation on the surface of the biofilm. Biofilm(BF) is forming bacteria and planktonic bacteria and the corresponding, in order to adapt to changes in the external environment of another growth pattern is a hybrid, continuous change, the dynamics of bacterial community, there is a certain space configuration of the bacterial population, is composed of DNA, RNA,fibrinogen, coated bacteria, bacterial lipopolysaccharide components.Due to biofilm formation, the bacterial resistance to antibiotics increased10 to 1000 times, to the clinical treatment of chronic infectious diseases caused by the problem. The research for adenoid whether biofilm formation, by detecting in the human innate immune response and specific immune response has an important role of TLR2 and TLR4 expression in children with adenoidal hypertrophy tissue, and cytokines IL-12 and IL-17 in adenoid hypertrophy in children serum content of therelationship between the biofilm and adenoid hypertrophy.Collected by 2015 may to 2016 January surgery excision of hypertrophic adenoid tissue and the corresponding serum 60 cases, serum of healthy children in 17 cases, by adenoid tissue bacterial isolation,culture, identification, divided into biomembrane group and non biological membrane group. The contents of IL-12 and IL-17 in the serum of children and healthy children were determined by ELISA method, and the expression of TLR2 and TLR4 were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)technique. The differences of IL-12 and IL-17 contents and the expression of TLR4 and TLR2 were compared between the groups.There were 27 cases of hypertrophic adenoid tissue in 60 cases, and33 cases without biofilm. Bacterial biofilm formation of Staphylococcus aureus, Streptococcus pneumoniae and Haemophilus influenzae, three kinds of bacteria in vitro culture 72 h after electron microscopy were able to form a clear biological membrane. Children with adenoid hypertrophy body serum IL- 12, IL- 17 content were higher than healthy children series(P < 0.05), there is a biofilm adenoid hypertrophy group of serum level of IL- 12 and no biological membrane group had no significant difference(P > 0.05), a biofilm adenoid hypertrophy group serum IL- 17 levels were significantly higher than those without a biofilm adenoid hypertrophy group(P< 0.05).All mast adenoid body tissues were detectedwith TLR2 and TLR4 expression, there’s a biofilm hypertrophy adenoidectomy in TLR2, TLR4 mRNA expression level with biofilm adenoid hypertrophy group no significant difference(P > 0.05, P > 0.05).To sum up the experiment we derive, Bacteria in hypertrophy adenoidectomy organization form a biofilm is one of the risk factors of adenoidectomy pathologic hypertrophy.IL-17 with adenoid hypertrophy and IL-12 are related, and the expression of IL-17 May in biofilm play a role in the onset of adenoid hypertrophy.Adenoid hypertrophy of body tissues TLR2 and TLR4 were higher, both free bacteria and biofilm formation expression had no effect on it.