Multi-Functional Gene Carriers And Antibacterial Materials Based On Glycidyl Methacrylate

It was well-known that gene therapy had been widely applied in cancers, cardiovascular diseases, and many other research areas. However, gene therapy also faced many obstacles and challenges in the process of use. The effect of treatment directly depends on the property of the gene carriers. As a result, multiple types of gene carriers had been reported. But most of non-viral gene carriers still have some drawbacks, including cytotoxicity, low transfection efficiency, bad biocompatibility and lack of multifunction. In recent years, the vectors based on were confirmed can effectively reduce the toxicity of gene carrier system. In addition, multi-functional gene carriers gradually got the attention of the researchers.In terms of bacterial infections, the drug resistance forced people to seek new types of antibacterial materials. Quaternary ammonium salt materials and silver ions had been proved to be active antibacterials. However, composite antibacterial materials showed better antibacterial effect. In addition, bacterias trended to adhere on surfaces and reproduce bacterial membranes. This phenomenon extended the treatment cycle of bacterial infection and largely increase the mortality rate of infected people. Surfaces modified by antibacterial materials exhibited a certain antibacterial and antiadhesion effect, and the quaternary ammonium salt polymers were commonly used. Grafted the antibacterial polymer chains on the surfaces was the common method used in the modification, But, the process of surface modification was complicated while the antibacterial effect was poor.In this paper, the first part of our work was to design protein-based multi-functional gene delivery systems. Firstly, the P-cyclodextrin (β-CD) was modified by 2-bromoisobutyryl bromide (BIBB) to produce a macroinitator CD-Br with four initiation sites. Then, CD-Br initiated glycidyl methacrylate for the typical ATRP polymerization to obtain poly(glycidyl methacrylate) (CD-PGMA) and after functionized by ethanol amine (EA), we got the CD-PGEA. Finally, through self-assembly function and electrostatic interaction, CD-PGEA was combined with adamantane modified bovine serum albumin (BSA-Ad) and Gd3+to form a new type of multifunctional gene delivery system with efficient magnetic resonance imaging (MRI) ability.The second work of this paper was to design antibacterial materials on the basis of the PGMA. Through the ring opening reactions of PGMA, we introduced the quaternary ammonium salt components to the PGMA polymer chains. After combined with silver ions components, a series of silver-quaternary ammonium salt cationic polymer composite antibacterial materials were produced. This kind of composite antibacterial materials have broad antibacterial activity for a variety of bacteria, and the minimal inhibitory concentration could be as low as 8 μg/mL. In addition, dopamine was confirmed to be able to formed poly (dopamine) (PDA) thin films on any surface in certain conditions. Therefore, the silver-quaternary ammonium salt cationic polymer composite antibacterial materials witch contained amino could graft on the surface of PDA modified glass to prepare new types of antibacterial surfaces with good antiadhesion and antibacterial effect.