The Applying Of Whole Exome Sequencing In Studying The Invasion Of Hepatocellular Carcinoma

Objective Hepatocellular carcinoma(HCC) is a common malignant tumor.It is the third major cause of cancer-related deaths worldwide.The occurrence of HCC related to tumor suppressor genes and(or) oncogenes mutation,but the pathogenesis of this cancer remains obscure. Whole exome sequencing(WES) is an economic and efficient method of sequencing in the identification of pathogenic genes of complex diseases. This experiment use WES to analyse tumor tissue and para-carcinoma tissue of different metastatic potential of HCC, try to identify the genetic mutations related to the occurrence, invasion and metastasis of HCC.Methods The 10 HCC divided into two groups : high metastatic potential group and low metastatic potential group, useing Complete Genomics(CG)Black Bird to sequence the DNA samples, and reference to database to call and filter useful informations. Find out the genetic mutations only exist in high metastatic potential group and tumor tissueResults 1.We identified 58 genes only exist in high metastatic potential group, including CCND3(rs1051130T/G)、CCRL2(rs3204849T/A)、CNTN2(rs2229866C/T)、FAT2(rs10044879G/A、rs150946802A/T*、rs2278370G/A)、MAP3K19(rs1112542G/C,rs16831235C/T)、PARP15(rs12489170G/A)、RECK(rs36108087G/A*,rs16932912G/A)、SDC1(rs10205485T/A)had been reported in some tumor,and frequent mutations of ADD1、ALDH2、FTSJ3 is worth to attention;2. There are 22 genes only exist in tumor tissue; including ADGB( rs147022152A/T* 、rs147045354G/T*)、 CTNNB1( rs121913412A/G)、 SAMD9L(rs92764797G/A*、rs92763885T/G*)had been reported that related to the occurrence of tumor.Conclusion 1. The mutations of CCND3、CCRL2、CNTN2、FAT2、MAP3K19、PARP15、RECK、SDC1 is related to the invasion and metastasis of HCC. 2.the mutations of ADD1、ALDH2、FTSJ3 may related to the invasion and metastasis of HCC,and needed to prove;3.the mutations of ADGB、CTNNB1、SAMD9L may related to the occurrence of HCC;...