Effect Of Acupuncture At Singal And Combined Acupoints On Antral Interstitial Cells Of Cajal In Diabetic Gastroparesis Model Rats

Purpose: To explore the effect of acupuncture at acupoint and combined acupoints on antral Interstitial Cells of Cajal(ICC)in diabetic gastroparesis model rats.Methods: 60 Sprague Dawley male rats were randomly divided into normal group,diabetic gastroparesis model rats group(model group),domperidone group,acupuncture at singal and combined acupoints an CV12,ST36,ST36+CV12,10 rats in each group.The rats were intraperitoneally injected with steptozotocin to make mouse model of diabetic gastroparesis.Treatments for each group were taken last for 5 weeks.And than,the numbers of ICC were detected by immunohistochemical methods.Results:After treatment,compared with normal group,Compared with normal group the number of ICC in model group were significantly reduced(P<0.01).Compared with model group,the indexes in Domperidone group(P<0.01)、acupuncture at ST36 and CV12 group(P<0.05),ST36+CV12 group(P<0.01)were improved significantly.Besides,the indexe of acupuncture at ST36+CV12 group was increased remarkably than ST36(P<0.05),CV12(P<0.01).Conclusion:Acupuncture could improve the numbers of antral Interstitial Cells of Cajal in diabetic gastroparesis model rats,acupuncture at combined acupoints was better than singal acupoint.