Expression Of Maspin In Squamous Cervical Carcinoma Of Transplanted In Nude Mice Tumor And Effect On Tumor Iymphangiogenesis

Objective:The objective of this study is to evaluate the effects of maspin overexpression on human cervical squamous carcinoma (SCC) SiHa cell proliferation and apoptosis in vitro, as well as the relationship between maspin and tumor lymphangiogenesis in nude mice.Methods:Recombinant plasmid pcDNA3-maspin was stably transfected into human cervical SCC SiHa cell. Maspin mRNA was determined using reverse transcription polymerase chain reaction (RT-PCR), whereas maspin protein was detected by Western blot analysis and immunohistochermistry (IHC). Cell proliferation activity was measured by MTT method. Apoptosis rate and cell cycle distribution were detected by flow cytometry to understand the changes in cell biological characteristics. The recombined SiHa cells were inoculated into six-week-old nude mice.The immunohistochemical method was used to detect the expression of maspin and podoplanin in nude mice tumor tissues. Statistical analysis was performed with rank-sum test and variance analysis.Results:The strengthened expression of the maspin gene in SiHa cell was confirmed by RT-PCR, Western blot, and IHC (P< 0.05). Suppressed proliferation activity and increased apoptosis rate of the pcDNA-maspin transfected SiHa cells (SiHa-m) versus SiHa and SiHa-vector cell (SiHa-pc3) were shown by MTT and flow cytometry (P< 0.05). The SiHa and SiHa-pc3 group had no statistical significance (P> 0.05). Tumor growth was significantly inhibited by recombinant plasmid pcDNA3-maspin in vivo. Statistical analysis showed that the SiHa-m group exhibited a significant difference in tumor volume compared with that of the SiHa and SiHa-pc3 group in weeks 1,3,4, and 5 (p< 0.05). No difference was observed between the SiHa and SiHa-pc3 group at different times (p> 0.05).Conclusions:Results showed that the maspin gene can significantly inhibit human cervical SCC SiHa cell proliferation and effectively reduce cancer growth. Maspin may be a new molecular target in the gene therapy of human cervical SCC.