Study On Extraction Of The Active Ingredient From Salvia Miltiorrhiza Bge. By Subcritical Water

Salvia miltiorrhiza Bge. is also called Bowley Sage Root, it is the dried root and rhizome of the labiatae plants Salvia miltiorrhiza. It has a very important position in the market of the traditional Chinese medicine and world’s medicinal materials and has the very long tradition in China. Because of the advent of Subcritical Water Extraction Technic, it has attracted a lot of attentions due to its short extraction time, low power consumption, high quality and high efficiency. Because of these advantages, Subcritical Water Extraction is viewed as the revolutionary technology which has the broad promising application and environmental friendly. The research utilizes subcritical water to extract the active ingredients in Salvia miltiorrhiza Bge. The HPLC method is used to measure the content. Taking Danshensu,protocatechual-dehyde, Salvianolic acid B, Cryptotanshione, anshinonel IIA as the assessment indexes, external standard method is used to calculate the extraction yield of these five active ingredients. We compare the different extraction methods by taking the extraction yield of different ingredients as the reference index. We also optimize the method to extract the active ingredients in Salvia miltiorrhiza Bge, trying to choose the best method to extract the active ingredients. This work can provide the theoretical basis for the revolution of traditional extraction technology and the scale production of Salvia miltiorrhiza Bge-related product.The thesis contains four chapters. The first chapter mainly introduces the Salvia miltiorrhiza Bge, the Subcritical Water Extraction method and setup, other extraction methods and the main content and significance of this research. In second chapter, by using sigle-factory-experiment, we study the influence of extraction time, extraction temperature, liquid-solid ratio and granularity on the extraction yield of fat-soluble and water-soluble ingredients. We also use the response surface methodology to optimize the extraction condition of active ingredients.The optimized conditions for Danshensu are 37.3min of extraction time, 220.5℃of extraction temperature, 1:15.5(g/ m L)of liquid-solid ratio. The optimized conditions for protocaterchual-dehyde are 32.4min of extraction time, 202 ℃ of extraction temperature,1:15.5(g/ m L) of liquid-solid ratio. The optimized conditions for Salvianolic acid B are9.5min of extraction time, 154℃ of extraction temperature, 1:20(g/ m L)of liquid-solid ratio.The optimized conditions for fat-soluble ingredients are 6.3min of extraction time, 165.5℃of extraction temperature, 1:10( g/ m L) of liquid-solid ratio. In chapter 3, ultrasound strengthen subcritical water extraction is induced and the influence of ultrasound time,ultrasound power and extraction temperature on the extraction yield of fat-soluble and wter-soluble ingredients. Comparing with the subcritical water extraction, Ultrasound strengthen subcritical water extraction can not only reduce the extraction temperature but also improve the extraction yield of the active ingredients. The chapter 4 compares the decocting method,the ultrasound extraction, reflux extraction, subcritical water extraction and ultrasound strengthen subcritical water extraction in terms of extraction process and extraction yield. The conclusion is subcritical water extraction and ultrasound strengthen subcritical water extraction exhibits shorter extraction time and environmental friendly comparing with other extraction methods.