Down-regulating Of RECQ1 Suppresses Non-small Cell Lung Cancer Invasion And Migration Via Inhibiting EMT And Clinical Analysis Of Lung Cancer Cases

Object: RECQ1,the most abundant one of the human RecQ helicases family,has been identified as a prometastasis gene in breast and cervical cancers.However,the effects of RECQ1 on non-small cell lung cancer(NSCLC)and the underlying molecular mechanisms are still unclear.Herein,lung cancer cases are analyzed to provide evidence for investigating whether radiographic examination has clinical significance in the early detection of lung cancer.Methods: RECQ1 expression(in three NSCLC cell lines and one bronchial epithelial cell line)was detected by real-time quantitative PCR(RT-qPCR).Expression of RECQ1 in A549 cells was knocked down by lentivirus-mediated RNA interference technique(RNAi).The effects of RECQ1 knockdown on cell proliferation,migration and invasion were assessed by Cell Counting Kit-8(CCK-8)assay and transwell assays.Epithelial-mesenchymal transition(EMT)-associated proteins(E-cadherin,N-cadherin as well as vimentin)were detected by RT-qPCR and western blotting analyses.The results of radiographic examination were compared with the pathological reports to discuss the veracity of radiographic examination in early lung cancer detection.Results: We found that RECQ1 expression was significantly higher in three NSCLC cell lines than that in a normal human bronchial epithelial cell line.Knocking down RECQ1 significantly suppressed A549 cell proliferation,migration and invasion.The expressions of the epithelial marker,E-cadherin were elevated in both mRNA and protein levels,whereas the expressions of the mesenchymal markers,N-cadherin andvimentin were decreased.In addition,radiographic examination could be considered as an effective method for early discovery of lung cancer.Conclusions: Our findings suggest that RECQ1 may act as an important mediator in promoting lung cancer progression via modulation of the EMT.RECQ1 might represent a potential therapeutic target in NSCLC.Our findings demonstrate the possibility of RECQ1 as a potential therapeutic target in NSCLC.Additionally,radiographic examination is of major importance in the early detection of lung cancer.