Vitro And Vivo Study On Functions Of CIKs Co-cultured With DCs Pulsed MCF-7 Breast Cancer Cell Freeze-thaw Antigen On The Cell Line Of MCF-7

Background Breast cancer has been one of the leading causes of cancer death in women.At present,treatment of breast cancer has developed different therapies such as surgery,chemotherapy,endocrine therapy,molecular target therapy and other treatment modalities.However,in some parts of the new cases of breast cancer patients,altimately metastasis and recurrence happened even after receiving adjuvant therapy in some patients.So it is still a challenging work for long-term treatment of breast cancer,and gives everyone pressure that looking for new treatment strategies for breast cancer.In recent years,study gradually found that it is possible to eliminate tumors,or prevent tumor from recurrence and metastasis by the identifying ability of immune system and method of regulating the immune response to tumor.But the application in treatments of breast cancer has not been fully recognized.In this study,we will study on the antitumor functions of cytokine induced killer cells(CIKs)co-cultured with dendritic cells(DCs)pulsed MCF-7 breast cancer cell freeze-thaw antigen on the cell line of MCF-7.ObjectiveTo investigate antitumor functions of cytokine induced killer cells(CIKs)co-cultured with dendritic cells(DCs)pulsed MCF-7 breast cancer cell freeze-thaw antigen on the cell line of MCF-7.To observe its effect on breast cancer cell activity,invasion ability and cell growth,so as to provide evidence for the new cell immunotherapy of breast cancer.MethodCulturing MCF-7 cells,preparing freeze-thaw antigen.Collect 50 ml peripheral venous blood of healthy people under strict aseptic conditions,extract the peripheral blood mononuclear cell(PBMC)into DC and CIK cells with cytokine recombinant human granulocyte-macrophage colony stimulating factor(rh GM-CSF),recombinant human Interleukin-4(rh IL-4),recombinant human interferon gamma(rh IFN-γ)and recombinant human Interleukin-2(rh IL-2).Promote DC cell maturation with TNF-alpha after being pulsed by MCF-7 breast cancer cell freeze-thaw antigen,and the combination of the two cell groups was performed after being.Make up the group.Analysis different effect of cytotoxic,invasion ability in vitro and tumorigenic ability in vivo.Results MCF-7 breast cancer cell freeze-thaw antigen can be cleaved and released under the condition of repeated freezing and thawing.In vitro,the CIK co-cultured with DC that pulsed with antigen present a best effect on cytotoxic activity with the optimal efficient ratio of 30:1(P < 0.05),every result was of difference in significance while it also present a best effect on reducing invasive effect(P < 0.05).In vivo,the average tumor size of DCMCF-7+CIK group was the minimum volume,which is(213±73)mm3.Tumor size in each group was of difference in significance(P < 0.05).Conclusion DC and CIK cells can be induced from peripheral blood mononuclear cells.By pulsing MCF-7 breast cancer cell freeze-thaw antigen,DC and CIK cells proves to have better performance about cytotoxic effect,significantly inhibited effect o n MCF-7 in vitro and impair the ability of MCF-7 cells to form tumor.