The Effect Of The Intravenous Anesthesia Induction On NK Cell Numbers And Subsets Of The Patients Without Underlying Disease

Objective: This study was performed to detect the numbers and surface subsets of NK cell(Nature killer cells)in peripheral blood of the young patients before and after the induction of intravenous anesthesia in young patients without underlying diseases(Basic metabolic disorders: such as endocrine disorders,diabetes;Immunocompromised: such as HIV/AIDS;Major chronic wasting disease: such as tumor).What we gonna do is to observe whether the changes of NK cell subsets are related to the induction of intravenous anesthesia and to explore the factors influencing of NK cell subsets and numbers.Method: 25 patients of ASA grade I~II without basic disease(Basic metabolic disorders: such as endocrine disorders,diabetes;Immunocompromised: such as HIV/AIDS;Major chronic wasting disease: such as tumor),Aged between 18 to 45,whom belongs to the department of ENT surgery,joint surge ry,oral and maxillofacial surgery were selected.After entering the operating room when the monitor was connected and all the vital signs of patients was showed completely,taking the peripheral blood samples 2ml in T0(before the induction of intravenous anesthesia),placed inside the vacuum tube containing anticoagulant;at the same time,recorded in pursuance of immediate systolic pressure,diastolic blood pressure,mean arterial pressure,heart rate and blood oxygen saturation.Then we start to use the general intravenous anesthesia drugs(penehyclidine hydrochloride injection 0.5mg,midazolam injection 0.05 mg.kg-1,etomidate injection 0.2-0.3 mg.kg-1,cisatracurium besilate for injection 0.2 mg.kg-1,sufentanil cireate injection 0.3-0.5 ug.kg-1)with the same standard for the induction of anesthesia.After the induction of intravenous anesthesia 10min(T1),taking the peripheral blood samples 2ml again at the same position,placed inside the vacuum tube containing anticoagulant,recorded in pursuance of immediate systolic pressure,diastolic blood pressure,mean arterial pressure,heart rate and blood oxygen saturation.The blood will be put in the tubes with anticoagulant and be stored in the refrigerator under the 4 degrees Celsius after mixing completely.Waiting for determining the changes related to the surface of NK cell subtypes(CD56 and CD16)through the Flow cytometry.)Result:(1)There are 25 cases of patients those whose basic vital signs changed: Comparing with T0,the systolic pressure,diastolic blood pressure,mean arterial pressure,and heart rate of T1 has changed greatly(P<0.01)with statistical significance.(2)Comparing with T0,the ratio of CD56 bright and CD 56+ CD16+ NK cell are declined.So as the experession of CD 16 +.This difference is significant(P<0.05),with statistical significance.And the proporation of the expression of CD56 dim an d CD 16-/ dim without significant difference.So it is no statistical significance.(3)Compa ring with T0,the level of decline of CD56 bright and CD 16+ NK cell at T1 were not significantly difference,P>0.05.Conclusion: The Intravenous induction of general anesthesia significant impact on the expression of CD16+ and CD56 bright,may in turn lead to decrease the number of NK cells and then affect the immune system function.