| Objective:To investigate the analgesic effect of opioid receptors involved in nuclear-volt of SD rats under CGRP. Methods:128 healthy male SD rats were used in weight of 250 ~ 300 g, after the experimental SD rats pawed at a temperature measuring instrument pain(hot plate) and pressure measuring instrument pain(plate), the training of male SD rats by the paw withdrawal response to painful stimuli, rats were treated with 40 mg / kg intraperitoneal injection of pentobarbital anesthesia. The use of stereotaxic instrument in SD rats Built into the nucleus accumbened 0.8mm diameter stainless steel tube. The experiments proceed as follows:1.The 32 male SD rats were randomly divided into four groups, each group 8, injected in the nucleus accumbens 1nmol / L of CGRP 1μl, 5 minutes later, the control group injected with saline 1μl, the experimental groups were injected concentration to 1nmol / L, 5nmol / L and 10 nmol / L naloxone each 1μl. After observing and recording rat paw response to the hot plate(thermal stimulation) and the platen(mechanical stimulation).2. The 32 male SD rats were randomly divided into four groups, each group 8, injected in the nucleus accumbens 1nmol / L of CGRP 1μl, 5 minutes later, the control group injected with saline 1μL, the experimental groups were injected concentration to 1nmol / L, 5nmol / L and 10 nmol / L of μ receptor antagonist β-FNA each 1μl. After observing and recording rat paw response to the hot plate(thermal stimulation) and the platen(mechanical stimulation).3.The 32 male SD rats were randomly divided into four groups, each group 8, injected in the nucleus accumbens 1 nmol /μL of CGRP 1μl, 5 minutes later, the control group injected with saline 1μl, the experimental groups were injected concentration respectively 1nmol / L, 5nmol / L and 10 nmo / L the δ receptor antagonist naltrindole each 1μl. After observing and recording rat paw response to the hot plate(thermal stimulation) and the platen(mechanical stimulation).4. The 32 male SD rats were randomly divided into four groups, each group 8, injected in the nucleus accumbens 1nmol / L of CGRP 1μl, 5 minutes later, the control group injected with saline 1μl, the experimental groups were injected concentration to 1nmol / L, 5nmol / L and 10 nmol / L the κ receptor antagonist nor-BNI each 1μl. After observing and recording rat paw response to the hot plate(thermal stimulation) and the platen(mechanical stimulation). Results:1.Compared with the control group injected with saline, there was significantly shorter(P <0.05) in paw response latency(HWL) of the experimental groups after injection of 5nmol / L and 10 nmol / L of naloxone, and there was no significant difference(P> 0.05) in paw response latency(HWL) of the experimental groups after injection of 1nmol / L the naloxone.2.Compared with the control group injected with saline, there was significantly shorter(P <0.05) in paw response latency(HWL) of the experimental groups after injection of 5nmol / L and 10 nmol / L of μ receptor antagonist β-FNA, and there was no significant difference(P> 0.05) in paw response latency(HWL) of the experimental groups after injection of 1nmol / Lμ receptor antagonist β- FNA.3.Compared with the control group injected with saline, there was no significant difference(P> 0.05) in paw response latency(HWL) of the experimental groups after injection of 1nmol / L, 5nmol / L and 10 nmol / L of δ receptor antagonist naltrindole.4.Compared with the control group injected with saline, there was significantly shorter(P <0.05) in paw response latency(HWL) of the experimental groups afterinjection of 5nmol / L and 10 nmol / L of the κ receptor antagonist nor-BNI, and there was no significant difference(P> 0.05) in paw response latency(HWL) of the experimental groups after injection of 1nmol / L of the κ receptor antagonist nor-BNI. Conclusions:The μ-opioid receptor and κ receptors receptors are involved in the calcitonin gene-related peptide(CGRP) and analgesic effects in the rat nucleus accumbens. |
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