| Objective: This study applies chain urea with cephalosporins(STZ) left lower quadrant abdominal injection preparation of diabetic gastroparesis(DGP)rat model, to give Chinese medicine Buzhong YiqiDecoction and mosapride citrate to the model,.We use the method of Westernblot to determine gastric antrum SCF protein expression, ria method to detect the plasma levels of SP.By observing the general situation of rats, gastric emptying rate, etc., we study the Buzhong YiqiDecoction on diabetic gastroparesis rate of gastric emptying, gastric antrum of rats SCF protein expression, the influence of plasma levels of SP, the discussion of Buzhong YiqiDecoction for DGP rats gastrointestinal function and possible mechanism, and provide the experimental basis for the TCM clinical treatment of DGP.Methods: We choose 40 clean level male wistar rats.Their weights swing between 180 g and 220 g. They purchased from hebei medical university animal center. First feed the experimental rats repurchase adaptive for one week, observe the general situation of rats, normal used in experiments. Take30 fasting 12 h, injecting chain urea with rhzomorph left abdomen STZ55.0mg/kg(PH = 4.2 with 0.1 M, sodium citrate, citric acid buffer solution mixture solution of 10 mg/mL), to observe a module appear more drinks, food,polyuria. We choose detection of fasting glucose tendency in 16.7 mL or more for diabetes model preparation, use high-energy food daily to eat in the morning, look at eating for disturbing rats eating rule, cause gastroparesis model. We record three groups of rats drinking, eating, urine, stool activity and change daily, measure blood glucose in the rat caudal vein for a week and weigh again. Randomly divided into Buzhong YiqiDecoction group, western medicine group, model control group, three groups, each 9, plus 9 fornon-diabetic normal blank group, a total of four groups. Groups began to fill medicine into the stomach after one week at the same time, the drug dose is:the normal group and model control group, normal saline 15ml/kg; Buzhong YiqiDecoction group of 15ml/kg, western medicine group of mosapride citrate5mg/kg, respectively to rats lavage, one time a day, to fill the stomach, a total of 7 weeks. Full 7 weeks after treatment, each group gives the semisolid paste(carboxymethyl cellulose by 2.6g, starch 2g,Milk powder 4g, 62.5 mL of distilled water), specific gravity 1.1 g.mL- 1. According to the 3mL·kg-1ig weight. Beheaded kill The rat after 30 min, then blood 5ml In the broken place to check level of SP,.Quickly open the rat abdominal cavity, ligature cardiac and pyloric, remove the stomach, use filter paper dry it and weigh its stomach full. Cut it along the greater curvature side of the appetizer, and rinse gastric contents repeatedly with distilled water, dry filter paper weighing stomach net weight. Press type calculation of gastric emptying, gastric emptying rate = [1-(stomach full weight- net weight)/ig quantity] x 100%. Take the distal tissue(100 mg) to extract the protein and measure SCF protein expression levels with Western Blot method.Results: 1 The general situation of the experimental rats:normal group rats have good mental state, activities, normal food intake, water volume and urine output, the weight is gradually increasing. Model group after building appears more drinking, eating, polyuria, weight lifting, skin dryness short-tempered yellowing. The general situation of traditional Chinese medicine, western medicine group rats are improving comparing with model group. In the process of test, a model group rats died,beause of improper building;2 Compared with the normal group, model group, Chinese medicine and western medicine group rats were significantly reduce weight, the difference was statistically significant(P< 0.05); Traditional Chinese medicine,western medicine group rats weight increased compared with model group, the difference was statistically significant(P< 0.05); Traditional Chinese medicine(TCM) weight increased than western medicine group there was statistically significant(P > 0.05);3 Model group, Chinese medicine, western medicinegroup rats blood glucose levels were significantly higher than that of normal group, the difference was statistically significant(P<0.05); Chinese traditional medicine group rats blood glucose levels compared with model group rats blood glucose decreased, the difference was statistically significant(P<0.05);The western medicine group, there was no statistically significant difference compared with model group blood sugar(P>0.05); Traditional Chinese medicine and western medicine group blood sugar difference was statistically significant(P < 0.05);4 Distal gastric smooth muscle tissue of rats wood grain- eosin(HE) staining showed that gastric antrum mucosa of rats in normal group organization structure is complete, rich blood vessels, glands, order more mesenchymal cells; Traditional Chinese medicine, western medicine group distal gastric mucous membrane is still intact, vessels with abundant,glandular arrangement is neat, mesenchymal cells number; Incomplete model group rats gastric antrum mucosa, blood vessel number decreased significantly,the disordered arrangement of glands and stroma cells decreases;5 Compared with normal group, model group, Chinese medicine, western medicine group rats gastric emptying rate decreased, the difference was statistically significant(P<0.05); Compared with model group, traditional Chinese medicine, western medicine group in the rat gastric emptying rate were significantly increased,the difference was statistically significant(P<0.05); Chinese traditional medicine than western medicine group gastric emptying rate is high, the difference was statistically significant(P<0.05); 6 Model group, Chinese medicine, western medicine group in the rat plasma SP level is lower than the normal group, the difference was statistically significant(P<0.05); Chinese traditional medicine group compared with model group rats plasma SP to reduce the difference was statistically significant(P<0.05); The western medicine group reduced plasma SP compared with model group was difference was statistically significant(P<0.05); Traditional Chinese medicine and western medicine group is elevated plasma levels of SP effect is no statistical significance(P>0.05);7 Model group, Chinese medicine, western medicine group rats gastric antrum SCF protein expression is lower thannormal group, the difference was statistically significant(P<0.05); Chinese traditional medicine group rats SCF protein expression reduces, compared with model group. The difference was statistically significant(P<0.05); The western medicine group compared with model group rats SCF protein expression to reduce the difference was statistically significant(P<0.05);Traditional Chinese medicine and western medicine group is elevated SCF protein expression effect was statistically significant(P<0.05).Conclusion:1 Diabetes gastroparesis rats have gastrointestinal dyskinesia, Buzhong YiqiDecoction can reduce DGP rats blood glucose level, increase the rate of rat gastric emptying, this can be one of the mechanism of action of the treatment of DGP.2 Buzhong YiqiDecoction to promote DGP rats treated rat gastric emptying, can improve the level of rat plasma SP, increase the gastrointestinal peristalsis, promote gastric emptying.3 Buzhong YiqiDecoction can improve the rat gastric antrum SCF protein expression, enhance SCF/KIT signaling, accelerate the gastrointestinal peristalsis, promote gastric emptying in the treatment of DGP, provides experiment basis for traditional Chinese medicine in the treatment of DGP. |
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